Abstract
Nicotiana rustica L. HZNH, a native Chinese tobacco germplasm, displays a hypersensitive response (HR) and systemic acquired resistance following infection with tobacco mosaic virus (TMV). A resistance gene, CN, cloned from HZNH plants, was homologous to the N and NH genes identified in other Nicotiana species. The CN coding region (3423 bp) shares 93.63% and 86.50% nucleotide identity with N and NH, respectively. Whereas the five CN exon sequences are highly homologous with those of N and NH, the four introns differ significantly in length and sequence. Sequence analysis revealed that CN belongs to the TIR/NBS/LRR gene class. Expression of CN was up-regulated after TMV infection and was temperature sensitive. Organ-specific expression analysis suggested that CN transcripts accumulated at high levels in leaves, low levels in stems, and minimal levels in roots. When CN was inserted into TMV-susceptible N. tabacum cv. K326 plants by Agrobacterium-mediated transformation, the transgenic plants displayed HR and systemic HR due to uninhibited movement of the virus.
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Acknowledgments
We are grateful to the State Tobacco Monopoly Administration for support of this work (110200101006). Thanks to Dr. Genji Qin for constructions of the overexpression vector and to Dr. Zhigang Xu for strain TMV-U1. We also thank Dr. R. A. McIntosh (Plant Breeding Institute, University of Sydney, Australia) for critical reading of the manuscript.
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Zhang, GY., Chen, M., Guo, JM. et al. Isolation and Characteristics of the CN Gene, a Tobacco Mosaic Virus Resistance N Gene Homolog, from Tobacco. Biochem Genet 47, 301–314 (2009). https://doi.org/10.1007/s10528-009-9229-3
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DOI: https://doi.org/10.1007/s10528-009-9229-3