Abstract
Genetic diversity of Monopterus albus in a wild population in the Sichuan Basin was investigated by employing mtDNA polymorphisms. The mitochondrial control region was sequenced for a total of 59 individuals collected from four localities. These sequences (553 bp) belonged to 13 haplotypes defined by eight polymorphic sites. The haplotypes were clustered into one lineage, suggesting that Monopterus albus from the Sichuan Basin might originate from a single ancestor in maternal lineages. The genetic differentiation between the population from Zhongxian in Chongqing city and the other three populations, from Mianyang, Longchang, and Ya’an in Sichuan province (F ST: 0.29167–0.55126), was higher than that among the latter three (F ST: −0.04196 to 0.05984). Little genetic differentiation was found in the three populations from Sichuan province (Nm: −6.21 to 3.93), and the Zhongxian population also showed lower genetic heterogeneity (P i: 0.00077 ± 0.00027; H d: 0.404 ± 0.130). The combination of limited variation and weak phylogenetic structure revealed that the Monopterus albus from the Sichuan Basin exhibited lower mtDNA diversity and should probably be treated as a monogroup genetically.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, USA
Cabe PR, Page RB, Hanlon TJ, Aldrich ME, Connors L, Marsh DM (2007) Fine-scale population differentiation and gene flow in a terrestrial salamander (Plethodon cinereus) living in continuous habitat. Heredity 98:53–60
Collins TM, Trexler J, Nico L, Rawlings TA (2002) Genetic diversity in a morphologically conservative invasive taxon: multiple introductions of swamp eels to the southeastern United States. Conserv Biol 16:1024–1035
He S, Liu X, Guo Z, Jin H (2004) On the genetic diversity of three species of Monopterus. J Hunan Agric Univ (Natural Sci) 30:145–147 (in Chinese with English abstract)
Hudson RR, Slatkin M, Maddison WP (1992) Estimation of levels of gene flow from DNA sequence data. Genetics 132:583–589
Li WT, Liao XL, Yu XM, Cheng L, Tong J (2007) Isolation and characterization of polymorphic microsatellites in a sex reversal fish, rice field eel (Monopterus albus). Mol Ecol Notes 7:705–707
Liu L, Wang W, Zheng B, Luo Y (2005) RAPD analysis of Monopterus albus (Zuiew) populations with three colors. Fisheries Sci 24:22–25 (in Chinese with English abstract)
Lu S, Liu S, Liu H, Liu Z, Liu Y (2005) Screening of microsatellite primer and its application to conservation genetics of Monopterus albus. J Fisheries China 29:612–618
Rozas J, Sánches-DelBarrio JC, Messeguer X, Rozas R (2003) DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics 19:2496–2497
Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236:787–792
Tamura K, Dudley J, Nei M, Kumar S (2007) Mega 4: Molecular Evolutionary Genetics Analysis (Mega) software version 4.0. Mol Biol Evol 24:1596–1599
Thai BT, Burridge CP, Pham TA, Austin CM (2004) Using mitochondrial nucleotide sequences to investigate diversity and genealogical relationships within common carp (Cyprinus carpio L.). Anim Genet 36:23–28
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgens DG (1997) The Clustal X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Res 25:4876–4882
Ward RD, Grewe PM (1994) Appraisal of molecular genetic techniques in fisheries. In: Pitcher TJ (ed) Molecular genetics in fisheries. Chapman & Hall, UK, pp 29–54
Yang F, Zhou Q, Zhang Y, Li X (2005) Variation of esterase isoenzyme among three kinds of bodily color Monopterus albus in Poyang Lake region. J Economic Anim 9:110–113 (in Chinese with English abstract)
Yin S, Li J, Zhou G, Liu Y (2005) Population genetic structure of rice field eel (Monopterus albus) with RAPD markers. Chin J Appl and Environ Biol 11:328–332 (in Chinese with English abstract)
Zhou R, Cheng H, Zhang Q, Guo Y, Richard RC, Terrence RT (2002) SRY-related genes in the genome of the rice field eel (Monopterus albus). Genet Sel Evol 34:129–137
Acknowledgments
We thank undergraduates Yao Lv, Lijun Feng, and Zhongyan Chen for their kind help with the experiments. This work was supported by the PhD Research Foundation of Southwest University of Science and Technology (no. 06ZX712).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cai, X., Gou, X., Zeng, F. et al. Mitochondrial DNA Diversity of Monopterus albus from the Sichuan Basin of China. Biochem Genet 46, 583–589 (2008). https://doi.org/10.1007/s10528-008-9172-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-008-9172-8