Abstract
CapZ is a widely distributed and highly conserved actin-binding protein that caps the barbed end of actin filaments and nucleates actin polymerization in a Ca2+-independent manner. In myofibrils, it is localized in the Z-lines. In this study, we cloned and characterized Capz subunit genes from the pig muscle. The nucleotide sequences and the predicted protein sequences share high sequence identity with other mammalian orthologs. The reverse transcriptase polymerase chain reaction (RT-PCR) revealed that porcine Capzβ, Capzα1, and Capzα2 genes are expressed in all 11 tissues studied (liver, spleen, small intestine, large intestine, lymph node, kidney, heart, skeletal muscle, brain, fat, and lung) but in variable amounts. Radiation hybrid mapping data indicated that Capzβ, Capzα1, and Capzα2 map to q2.1–2.6 of pig chromosome 6 (SSC6), q1.6–q2.2 of pig chromosome 4 (SSC4), and q1.3–2.3 of pig chromosome 18 (SSC18), respectively. An A/C single nucleotide polymorphism in Capzβ intron 4 was identified with a HhaI PCR restriction fragment length polymorphism, which showed great allele frequency differences between Guizhou Xiang, Guangxi Bama, Wuzhishan, Tongcheng, Landrace, and Yorkshire pigs. The association analysis suggested that the Capzβ genotype was associated with leaf fat (P < 0.05) in our experimental population.
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Acknowledgments
The authors wish to express their thanks to Dr Martine Yerle of INRA for providing the RH panel. This research was supported by the National High Science and Technology Foundation of China (20060110Z1039), National Natural Science Foundation of China (30500359), Key Project of National Basic Research and Developmental Plan of China (G2006CB102105), Key Project of National Natural Science of China (30330440), State Platform of Technology Infrastructure (2005DKA21101), and Key Project of Scientific Research Foundation of Ministry of Human Resources of China for Returned Chinese Scholars.
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Yang, E., Wang, H., Wu, X.X. et al. Sequence Characterization, Polymorphism, and Chromosomal Localizations of the Porcine Capz Genes. Biochem Genet 46, 18–28 (2008). https://doi.org/10.1007/s10528-007-9125-7
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DOI: https://doi.org/10.1007/s10528-007-9125-7