Abstract
By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA (C4orf13). This cDNA is 2706 bp in length, encoding a 340-amino-acid polypeptide that contains a typical SBF (sodium bile acid cotransporter family) domain and ten possible transmembrane segments. The putative protein C4orf13 shows high similarity with its orthologs in Mus musculus and Xenopus laevis. Human C4orf13 is mapped to chromosome 4q31.2 and contains 12 exons. RT-PCR analysis shows that human C4orf13 is widely expressed in human tissues, and the expression levels in liver and lung are relatively high, expression levels in placenta, kidney, spleen, and thymus are moderate, low levels of expression are detected in heart, prostate, and testis.
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References
Alcalay, M., and Toniolo, D. (1988). CpG islands of the X chromosome are gene associated. Nucleic acids Res. 16(20):9527–9543.
Bobrowicz, P., Wysocki, R., Owsianik, G., Goffeau, A., and Ulaszewski, S. (1997). Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae. Yeast 13:819–828.
Craddock, A. L., Love, M. W., Daniel, R. W., Kirby, L. C., Walters, H. C., Wong, M. H., and Dawson, P. A. (1998). Expression and transport properties of the human ileal and renal sodium-dependent bile acid transporter. Am. J. Physiol. 274:G157–G169.
Hagenbuch, B., Stieger, B., Foguet, M., Lubbert, H., and Meier, P. J. (1991). Functional expression cloning and characterization of the hepatocyte Na+/bile acid cotransport system. Proc. Natl. Acad. Sci. U.S.A. 88:10629–10633.
Hofmann, A. F. (1993). In Sleisenger, M. H., and Fordtran, J. S. (eds.), Gastrointestinal Disease: Pathophysiology/Diagnosis/Management, Saunders, Philadelphia, PA, pp. 127-150.
Hofmann, A. F. (1994). Bile acids. In Arias, I. M., Boyer, J. L., Fausto, N., Jakoby, W. B., Schachter, D. A., and Shafritz, D. A. (eds.), The Liver: Biology and Pathobiology, Raven, New York, pp. 677–717.
Russell, D. W., and Setchell, K. D. R. (1992). Bile acids biosynthesis, Biochemistry 31:4737–4749.
Shiao, T., Lwahashi, M., Fortune, J., Quattrochi, L., Bowman, S., Wick, M., Qadri, I., and Simon, F. R. (2000). Structural and functional characterization of liver cell-specific activity of the human sodium/taurocholate cotransporter. Genomics 69:203–213.
Weiner, I. M., Glasser, J. E., and Lack, L. (1964). Renal excretion of bile acids: taurocholate, glycocholate, and cholic acids. Am. J. physiol. 207:964–970.
Wilson, F. A. G., Burckhardt, G., Murer, H., Rumrich, G., and Ullrich, K. J. (1981). Sodium-coupled taurocholate transport in the proximal convolution of the rat kidney in vivo and in vitro. J. Clin. Invest. 67:1141–1150.
Wong, M. H., Oelkers, P., and Dawson, P. A. (1995). Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity, J. Biol. Chem. 270(45):27228–27234.
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The nucleotide sequence reported in this paper has been deposited to GenBank under accession number AY346324.
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Zou, X., Wang, D., Qiu, G. et al. Molecular Cloning and Characterization of a Novel Human C4orf13 Gene, Tentatively a Member of the Sodium Bile Acid Cotransporter Family. Biochem Genet 43, 165–173 (2005). https://doi.org/10.1007/s10528-005-1509-y
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DOI: https://doi.org/10.1007/s10528-005-1509-y