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Experimental studies of semiautomatic cryoconservation process for biological tissue specimens

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Abstract

Local low-temperature effects on samples of sheep aorta were studied using an experimental apparatus serving as a prototype for a medical device for semi-automatic cryopreservation of biological tissue with the option of using a variety of cryoprotectants with regulation of coolant flow and temperature. The effectiveness of cell preservation was assessed by histological examination. The results showed that use of polydimethylsiloxane as a cryoprotectant led to preservation of biological tissue and has prospects for further research in order to find optimal modes of semi-automatic cryopreservation.

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Acknowledgements

This study was supported by a grant from the Russian Science Foundation (project no. 21-19-00676).

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Authors and Affiliations

  1. Department of Refrigeration and Cryogenic Technology, Conditioning Systems, and Life Support Systems, Bauman Moscow State Technical University (A National Research University), Moscow, Russian Federation

    A. V. Pushkarev, I. A. Burkov, V. M. Ivannikova, N. Yu. Saakyan & D. I. Tsiganov

  2. Department of Medical Technology, Russian Medical Academy for Continuing Professional Education, Russian Federation Ministry of Health, Moscow, Russian Federation

    A. V. Pushkarev & D. I. Tsiganov

  3. Cryogenic Technologies in Energy Engineering Center, Bauman Moscow State Technical University (A National Research University), Moscow, Russian Federation

    A. S. Krotov

Authors
  1. A. V. Pushkarev
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  2. I. A. Burkov
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  3. V. M. Ivannikova
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  4. N. Yu. Saakyan
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  5. A. S. Krotov
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  6. D. I. Tsiganov
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Corresponding author

Correspondence to A. V. Pushkarev.

Additional information

Translated from Meditsinskaya Tekhnika, Vol. 57, No. 6, pp. 17–19, November-December, 2023. Original article submitted October 13, 2023.

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Pushkarev, A.V., Burkov, I.A., Ivannikova, V.M. et al. Experimental studies of semiautomatic cryoconservation process for biological tissue specimens. Biomed Eng (2024). https://doi.org/10.1007/s10527-024-10341-y

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  • DOI: https://doi.org/10.1007/s10527-024-10341-y

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