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Effect of wax degrading bacteria on life cycle of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae)

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Abstract

The pink mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) is a polyphagous insect pest with protective wax covering. Bacterial isolates originating from M. hirsutus cadavers were screened for their wax degrading effects and the three most potent wax degrading isolates were identified to be Serratia marcescens, Pseudomonas aeruginosa and Bacillus subtilis by 16S rRNA gene sequencing. Bacteria degrading M. hirsutus wax reduced female longevity, offspring production as well as weight and wax content of emerging adults. Treatment with S. marcescens was the most effective in reducing wax content, fecundity and honeydew production. Analysis of honeydew produced by M. hirsutus after each microbial treatment revealed reduction in amino acids, proteins, and reducing sugars concentration compared to controls. Most negative effect on reducing sugars was observed in the B. subtilis treatment suggesting disequilibrium in mealybug metabolism. S. marcescens and P. aeruginosa treatments showed significantly lower content of wax as compared to control suggesting utilization of wax as carbon source by these microorganisms. This study suggests that the use of each of three bacterial isolates reduces wax content of mealybug and might be useful in devising mealybug control strategies.

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Acknowledgments

The Financial assistance to Dr. Satish V. Patil in the form Project Grant File No. (BT/PR12694/AGR/05/474/(letter II) dated 30 June 2010) from Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India is gratefully acknowledged.

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Authors and Affiliations

  1. School of Life Sciences, North Maharashtra University, Post Box-80, Jalgaon, 425001, Maharashtra, India

    Rahul B. Salunkhe, Chandrashekhar D. Patil, Bipinchandra K. Salunke & Satish V. Patil

  2. Laboratorio de Biotecnología Ambiental, Centro de Biotecnología, Genómica-Instituto Politécnico Nacional, Blvd. del Maestro s/n, Col. Narciso Mendoza, CP. 88710, Reynosa, Tamaulipas, Mexico

    Ninfa M. Rosas-García

  3. North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, Post Box-80, Jalgaon, 425001, Maharashtra, India

    Satish V. Patil

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  1. Rahul B. Salunkhe
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  2. Chandrashekhar D. Patil
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  3. Bipinchandra K. Salunke
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Correspondence to Satish V. Patil.

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Salunkhe, R.B., Patil, C.D., Salunke, B.K. et al. Effect of wax degrading bacteria on life cycle of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). BioControl 58, 535–542 (2013). https://doi.org/10.1007/s10526-013-9513-3

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  • DOI: https://doi.org/10.1007/s10526-013-9513-3

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