Skip to main content
SpringerLink
Log in

Effect of temperature on the in vitro radial growth of Zoophthora radicans and Pandora blunckii, two co-occurring fungal pathogens of the diamondback moth Plutella xylostella

  • Published:
BioControl Aims and scope Submit manuscript

Abstract

The effect of four different temperatures (15, 20, 25 and 30°C) on the in vitro growth of 19 isolates of Pandora blunckii and 14 isolates of Zoophthora radicans from Plutella xylostella larvae was investigated. Both species grew more at 20 and 25°C than the other two temperatures. However, Z. radicans grew more than P. blunckii at 20 and 25°C. Within each species there were differences amongst: all isolates regardless of geographical origin, isolates from different countries and isolates from Mexico. No relationship was found between optimal growth temperature and geographical origin. This represents the first report of the relationship between temperature and the in vitro growth of P. blunckii. The ecological role of this large variability amongst isolates within each species is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Benz G (1987) Environment. In: Fuxa JR, Tanada Y (eds) Epizootiology of insect diseases. Wiley, NY, pp 177–214

    Google Scholar 

  • Bidochka MJ, Kamp AM, Lavender M, Dekoning J, De Croos JNA (2001) Habitat association in two genetic groups of the insect-pathogenic fungus Metarhizium anisopliae: uncovering cryptic species? Appl Environ Microbiol 67(3):1335–1342

    Article  PubMed  CAS  Google Scholar 

  • Blanford S, Thomas MB, Langewald J (2000) Thermal ecology of Zonocerus variegatus and its effects on biocontrol using pathogens. Agric For Entomol 2:3–10

    Article  Google Scholar 

  • Cooke RC, Rayner ADM (1984) Ecology of saprothropic fungi. Longman Group, England

    Google Scholar 

  • Cooke RC, Whipps JM (1993) Ecophysiology of fungi. Blackwell Scientific Publications, Cambridge

    Google Scholar 

  • Deacon JW (1997) Modern mycology, 3rd edn. Blackwell Science, Cambridge

    Google Scholar 

  • Dimbi S, Maniana NK, Luz SA, Mueke JM (2004) Effect of constant temperatures on germination, radial growth and virulence of Metarhizium anisopliae to three species of African tephritid fruit flies. BioControl 49:83–94

    Article  Google Scholar 

  • Ekesi S (1999) Selection of virulent isolates of entomopathogenic hyphomycetes against Clavigralla tomentosicollis Stal. and evaluation in cage experiment using three cowpea varieties. Mycopathologia 148(3):131–139

    Article  PubMed  CAS  Google Scholar 

  • Fargues J, Goettel MS, Smits N, Ouedraogo A, Rougier M (1997) Effect of temperature on vegetative growth of Beauveria bassiana isolates from different origins. Mycologia 89(3):383–392

    Article  Google Scholar 

  • Fargues J, Maniania NK, Delmas JC, Smits N (1992) Influence de la température sur la croissance in vitro d’hyphomycètes entomopathogènes. Agronomie 12:557–564

    Article  Google Scholar 

  • Feng MG, Johnson JB (1991) Bioassay of four entomophthoralean fungi (Entomophthorales) against Diuraphis noxia and Metopolophium dirhodum (Homoptera: Aphididae). Environ Entomol 20(1):338–345

    Google Scholar 

  • Furlong MJ, Pell JK (1997) The influence of environmental factors on the persistence of Zoophthora radicans conidia. J Invertebr Pathol 69:223–233

    Article  Google Scholar 

  • Genstat 6 Committee (2000) Genstat 6 release 3 reference manual. Oxford University Press, Oxford

    Google Scholar 

  • Glare TR, Milner RJ, Chilvers GA, Mahon RJ, Brown WV (1987) Taxonomic implications of intraspecific variation amongst isolates of the aphid-pathogenic fungi Zoophthora radicans Brefeld and Z. phalloides Batko (Zygomycetes: Entomophthoraceae). Aust J Bot 35:49–67

    Article  Google Scholar 

  • Griggs MH, Vandenberg JD, Sawyer AJ (1999) Effect of relative humidity on variability of primary conidia of Zoophthora radicans. J Invertebr Pathol 73:315–320

    Article  PubMed  CAS  Google Scholar 

  • Inglis GD, Johnson DL, Goettel MS (1996) Effects of temperature and thermoregulation on mycosis by Beauveria bassiana in grasshoppers. Biol Control 7:131–139

    Article  Google Scholar 

  • Kenward MG (1987) A method for comparing profiles of repeated measurements. Appl Stat 36:296–308

    Article  Google Scholar 

  • Milner RJ, Lutton GG (1983) Effect of temperature on Zoophthora radicans (Brefeld) Batko: an introduced microbial control agent of the spotted alfalfa aphid, Therioaphis trifolii (Monell) f. maculate. J Aust Entomol Soc 22:167–173

    Article  Google Scholar 

  • Milner RJ, Mahon RJ (1985) Strain variation in Zoophthora radicans, a pathogen on a variety of insect hosts in Australia. J Aust Entomol Soc 24:195–198

    Article  Google Scholar 

  • Moorhouse ER, Gillespie AT, Charnley AK (1994) The influence of temperature on the susceptibility of vine weevil, Otiorhynchus sulcatus (Fabricius) (Coleoptera: Curculionidae), larvae to Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). Ann Appl Biol 124:185–193

    Article  Google Scholar 

  • Morgan LW, Boddy L, Clark SJ, Wilding N (1995) Influence of temperature on germination of primary and secondary conidia of Erynia neoaphidis (Zygomycetes: Entomophthorales). J Invertebr Pathol 65:132–138

    Article  Google Scholar 

  • Pell JK, Wilding N, Player AL, Clark SJ (1993) Selection of an isolate of Zoophthora radicans (Zygomycetes: Entomophthorales) for biocontrol of the diamondback moth Plutella xylostella (Lepidoptera: Yponomeutidae). J Invertebr Pathol 61:75–80

    Article  Google Scholar 

  • Pell JK, Eilenberg J, Hajek AE, Steinkraus DS (2001) Biology, ecology and pest management potential of entomophthorales. In: Butt TM, Jackson C, Magan N (eds) Fungi as biocontrol agents: progress, problems and potential, vol 390. CABI International, Wallingford, UK, pp 71–154

  • Riethmacher GW, Kranz J (1994) Development of disease incidence of entomophthoraceae in field populations of Plutella xylostella in the Philippines. J Plant Dis Prot 101(4):357–367

    Google Scholar 

  • Shelton AM, Perez CJ, Tang JD, Vandenberg JD (1997) Prospects for novel approaches towards management of the diamondback moth. In: Sivapragasm A, Loke WH, Hussan AK, Lim GS (eds) Proceedings of the 3rd international workshop on the management of diamondback moth and other crucifer pests. Kuala Lumpur, Malaysia, pp 17–20

    Google Scholar 

  • Stacey DA, Thomas MB, Blanford S, Pell JK, Pugh C, Fellowes MD (2003) Genotype and temperature influence pea aphid resistance to a fungal entomopathogen. Physiol Entomol 28:75–81

    Article  Google Scholar 

  • Tanada Y, Kaya HK (1993) Insect pathology. Academic, CA, USA

    Google Scholar 

  • Tefera T, Pringle K (2003) Germination, radial growth, and sporulation of Beauveria bassiana and Metarhizium anisopliae isolates and their virulence to Chilo partellus (Lepidoptera: Pyralidae) at different temperatures. Biocontrol Sci Technol 13(7):699–704

    Article  Google Scholar 

  • Thomas MB, Jenkins NE (1997) Effects of temperature on growth of Metarhizium flavoviridae and virulence to the variegated grasshopper, Zonocerus variegates. Mycol Res 101(12):1469–1474

    Article  Google Scholar 

  • Tomiyama H, Aoki J (1982) Infection of Erynia blunckii (Lak. Ex Zimm.) Rem. Et Henn. (Entomophthorales: Entomophthoraceae) in the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae). Appl Entomol Zool 17(3):375–384

    Google Scholar 

  • Uziel A, Shtienberg D (1993) Effect of meteorological variates on persistence of primary conidia and capilliconidia of Erynia radicans (Zygomycotina: Entomophthorales) under natural conditions. Ann Appl Biol 122:441–450

    Article  Google Scholar 

  • Velasco-Silva J, Alatorre-Rosas R, Pell JK, Guzmán-Franco A (2000) Characterization of native entomophthoralean fungi associated with Plutella xylostella (Lepidoptera: Plutellidae) in the Bajio region, Guanajuato, México. In: Abstracts of the 33rd annual meeting of the society for invertebrate pathology. University of Guanajuato, México, p 97

  • Vidal C, Fargues J, Lacey LA (1997) Intraspecific variability of Paecilomyces fumosoroseus: effect of temperature on vegetative growth. J Invertebr Pathol 70:18–26

    Article  Google Scholar 

  • Wilding N (1986) The pathogens of diamondback moth and their potential for its control—a review. In: Talekar NS, Griggs TD (eds) Diamondback moth management. Proceedings of the 1st international workshop, Tainan, Taiwan, 11–15 March 1985. Asian Vegetable Research and Development Center, Taiwan, pp 219–232

  • Wilding N, Brobyn PJ (1980) Effects of fungicides on the development of Entomophthora aphidis. Trans Br Mycol Soc 75:279–302

    Article  Google Scholar 

  • Yeo H, Pell JK, Alderson PG, Clark SJ, Pye BJ (2003) Laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species. Pest Manag Sci 59:156–165

    Article  PubMed  CAS  Google Scholar 

  • Yeo H, Pell JK, Walter M, Boyd-Wilson KSH, Snelling C, Suckling DM (2001) Susceptibility of diamondback moth (Plutella xylostella (L.)) larvae to the entomopathogenic fungus Zoophthora radicans (Brefeld) Batko. NZ Plant Prot 54:47–50

    Google Scholar 

Download references

Acknowledgements

AGF was supported by Asociacion Nacional de Investigaciones e Instituciones de Educación Superior (ANUIES) and Colegio de Postgraduados, México. JKP was supported by the Department for Environment, Food and Rural Affairs of UK (Defra). Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of UK.

Author information

Author notes

  1. Ariel W. Guzmán-Franco

    Present address: Instituto de Fitosanidad, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de Mexico, 56230, Mexico

Authors and Affiliations

  1. Centre for Soils and Ecosystem Function, Plant and Invertebrate Ecology Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    Ariel W. Guzmán-Franco & Judith K. Pell

  2. School of Biosciences, University of Nottingham, Loughborough, Leicestershire, LE12 5RD, UK

    Ariel W. Guzmán-Franco & Peter G. Alderson

  3. Centre for Mathematical and Computational Biology, Department of Biomathematics and Bioinformatics, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK

    Suzanne J. Clark

Authors
  1. Ariel W. Guzmán-Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suzanne J. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter G. Alderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Judith K. Pell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Judith K. Pell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guzmán-Franco, A.W., Clark, S.J., Alderson, P.G. et al. Effect of temperature on the in vitro radial growth of Zoophthora radicans and Pandora blunckii, two co-occurring fungal pathogens of the diamondback moth Plutella xylostella . BioControl 53, 501–516 (2008). https://doi.org/10.1007/s10526-007-9095-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10526-007-9095-z

Keywords

Search

Navigation