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Stratification of yeast cells during chronological aging by size points to the role of trehalose in cell vitality

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Abstract

Cells of the budding yeast Saccharomyces cerevisiae undergo a process akin to differentiation during prolonged culture without medium replenishment. Various methods have been used to separate and determine the potential role and fate of the different cell species. We have stratified chronologically-aged yeast cultures into cells of different sizes, using centrifugal elutriation, and characterized these subpopulations physiologically. We distinguish two extreme cell types, very small (XS) and very large (L) cells. L cells display higher viability based on two separate criteria. They respire much more actively, but produce lower levels of reactive oxygen species (ROS). L cells are capable of dividing, albeit slowly, giving rise to XS cells which do not divide. L cells are more resistant to osmotic stress and they have higher trehalose content, a storage carbohydrate often connected to stress resistance. Depletion of trehalose by deletion of TPS2 does not affect the vital characteristics of L cells, but it improves some of these characteristics in XS cells. Therefore, we propose that the response of L and XS cells to the trehalose produced in the former differs in a way that lowers the vitality of the latter. We compare our XS- and L-fraction cell characteristics with those of cells isolated from stationary cultures by others based on density. This comparison suggests that the cells have some similarities but also differences that may prove useful in addressing whether it is the segregation or the response to trehalose that may play the predominant role in cell division from stationary culture.

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Acknowledgments

The work was supported by the Grant LH13049 of the CR Ministry of Education, Youth and Sports, internal institutional project RVO 61388971 of Institute of Microbiology Acadamy of Sciences of the Czech Republic and by Grant AG006168 from the National Institute on Aging of the National Institutes of Health (U.S.P.H.S.) to SMJ. We acknowledge the Cytometry and Microscopy Facility at the Institute of Microbiology Acadamy of Sciences of the Czech Republic, v.v.i, Videňska 1083, Prague, CZ for the use of cytometry equipment and the support from the staff.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Andrea Svenkrtova, Lenka Belicova, Andrea Volejnikova, Karel Sigler & Alena Pichova

  2. Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Prague, Czech Republic

    Andrea Svenkrtova, Lenka Belicova & Andrea Volejnikova

  3. Department of Medicine, Tulane Center for Aging, Tulane University Health Sciences Center, New Orleans, LA, USA

    S. Michal Jazwinski

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  1. Andrea Svenkrtova
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  2. Lenka Belicova
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  3. Andrea Volejnikova
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  4. Karel Sigler
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  5. S. Michal Jazwinski
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  6. Alena Pichova
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Correspondence to Alena Pichova.

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Svenkrtova, A., Belicova, L., Volejnikova, A. et al. Stratification of yeast cells during chronological aging by size points to the role of trehalose in cell vitality. Biogerontology 17, 395–408 (2016). https://doi.org/10.1007/s10522-015-9625-5

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  • DOI: https://doi.org/10.1007/s10522-015-9625-5

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