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The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma

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Bulletin of Experimental Biology and Medicine Aims and scope

The role of methylation of 9 miRNA genes in the pathogenesis of metastatic clear cell renal cell carcinoma was determined by quantitative methylation-specific PCR (MS-PCR). For 5 genes (MIR125B-1, MIR137, MIR193A, MIR34B/C, and MIR375), a significant correlation of high methylation level with late (III-IV) stages, large size (T3+T4) of the tumor, and metastasis to lymph nodes and/or distant organs was revealed. For another group of genes (MIR125B-1, MIR1258, MIR193A, MIR34B/C, and MIR375), a statistically significant correlation of high methylation level with loss of differentiation in the tumor (G3-G4) was found, and the opposite pattern was found for MIR203A. A total of 7 microRNA genes (MIR125B-1, MIR1258, MIR137, MIR193A, MIR203A, MIR34B/C, and MIR375) were identified, the methylation of which is associated with the progression of metastatic clear cell renal cell carcinoma. For 6 of them (except MIR34B/C) these data were obtained for the first time. Thus, new factors of the development and progression of clear cell renal cell carcinoma were identified as potential biomarkers for the early diagnosis and prognosis of metastatic clear cell renal cell carcinoma.

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, Moscow, Russia

    N. A. Ivanova, A. M. Burdennyi, S. S. Lukina, E. A. Filippova, I. V. Pronina, V. I. Loginov & E. A. Braga

  2. N. P. Bochkov Research Center for Medical Genetics, Moscow, Russia

    A. V. Karpukhin

  3. N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia

    V. B. Matveev, T. P. Kazubskaya & N. E. Kushlinskii

Authors
  1. N. A. Ivanova
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  2. A. M. Burdennyi
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  3. S. S. Lukina
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  4. E. A. Filippova
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  5. I. V. Pronina
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  6. A. V. Karpukhin
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  7. V. B. Matveev
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  8. T. P. Kazubskaya
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  9. V. I. Loginov
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  10. E. A. Braga
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  11. N. E. Kushlinskii
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Corresponding author

Correspondence to A. M. Burdennyi.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 2, pp. 215-219, February, 2023

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Ivanova, N.A., Burdennyi, A.M., Lukina, S.S. et al. The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma. Bull Exp Biol Med 175, 249–253 (2023). https://doi.org/10.1007/s10517-023-05844-9

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  • DOI: https://doi.org/10.1007/s10517-023-05844-9

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