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The Use of Neural Stem Cells-Derived Exosomes to Prevent Late Radiation-Induced Cognitive Impairments in Mice

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of intranasal administration of neural stem cell (NSC)-derived exosomes on behavior and cognitive functions of mice in the late period after head irradiation in a dose of 8 Gy. The used exosomes had specific markers (CD9+/CD63+, 99.5%; TSG101+, 98.4%) and mean size 105.7±8.8 nm according to dynamic light scattering data and 119.0±12.4 nm according to nanoparticle tracking analysis (NTA). Exosome suspension (2×1012 particles/ml according to NTA measurements) was administered intranasally for 4 weeks starting from 48 h after irradiation in a volume of 5 μl/nostril (2×1010 exosomes/mouse). It was shown that intranasal administration of mouse NSC-derived exosomes prevented delayed radiation-induced behavioral changes and recognition memory impairments in mice after head irradiation.

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Authors and Affiliations

  1. National Research Center “Kurchatov Institute”, Moscow, Russia

    M. G. Ratushnyak, A. S. Zhirnik, O. D. Smirnova, Yu.P. Semochkina, A. A. Parfenova & E. Yu. Moskaleva

  2. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    K. V. Goryunov & D. N. Silachev

Authors
  1. M. G. Ratushnyak
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  2. A. S. Zhirnik
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  3. O. D. Smirnova
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  4. Yu.P. Semochkina
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  5. A. A. Parfenova
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  6. K. V. Goryunov
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  7. D. N. Silachev
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  8. E. Yu. Moskaleva
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Corresponding author

Correspondence to M. G. Ratushnyak.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 261-268, December, 2022

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Ratushnyak, M.G., Zhirnik, A.S., Smirnova, O.D. et al. The Use of Neural Stem Cells-Derived Exosomes to Prevent Late Radiation-Induced Cognitive Impairments in Mice. Bull Exp Biol Med 174, 571–577 (2023). https://doi.org/10.1007/s10517-023-05749-7

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  • DOI: https://doi.org/10.1007/s10517-023-05749-7

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