We studied the effect of xenon on the survival rate of the spermatozoa of the common frog Rana temporaria during slow freezing with saturation of the suspension with xenon at a pressure of up to 1.2 bar. The cryoprotective properties of xenon were analyzed in comparison with nitrogen. No specific cryoprotective effect of xenon was revealed. Viability of spermatozoa pretreated with xenon at atmospheric pressure (0 bar) or under excess pressure of 0.6 bar and frozen in a cryoprotective medium with dimethylformamide, sucrose, and BSA did not differ significantly. The use of overpressure of xenon of 1.0 or 1.2 bar in the pretreatment and freezing process significantly impaired viability of the biomaterial.
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Laptev DS, Polezhaeva TV, Zaitseva OO, Khudyakov AN, Utemov SV, Knyazev MG, Kostyaev AA. Effect of inert gas xenon on the functional state of nucleated cells of peripheral blood during freezing. Human Physiol. 2015;41(2):206-208.
Makeev OG, Ponomarev AI, Korotkov AV. The application of a clathrate forming gas for cryopreservation of multipotent mesenchymal stromal cells. Vestn. Ural. Med. Akad. Nauki. 2010;(1):52-54. Russian.
Manakov AY, Rodionova TV, Penkov NV, Fesenko EE, Nesterov AN. Kinetics of formation and dissociation of gas hydrates. Russ. Chem. Rev. 2017;86(9):845-869.
Ponomarev AI, Makeev OG, Zvereva AE, Korotkov AV. Applicability of the xenon clathrates protocol for skin grafts preservation. Vestn. Ural. Med. Akad. Nauki. 2014;(5):98-102. Russian.
Pulver AY, Tselikovsky AV, Pulver NA, Artyukhov IV, Peregudov AG. Cryopreservation of mammalian cell cultures using xenon. Theoretical and practical aspects of modern cryobiology. Materials of the International Correspondence Scientific and Practical Conference (March 24, 2014 Russia-Ukraine) Syktyvkar. 2014. P. 226-232. Russian.
Khlusov IA, Zhukov PG, Sukhikh GT. Cell effects of xenon in vitro under hypothermal conditions. Bull. Exp. Biol. Med. 2007;143(4):510-513. doi: https://doi.org/10.1007/s10517-007-0168-x
Shishova NV, Fesenko EE. The prospects of the application of gases and gas hydrates in cryopreservation. Biophysics. 2015;60(5):782-804.
Artyukhov VI, Pulver AY, Peregudov A, Artyuhov I. Can xenon in water inhibit ice growth? Molecular dynamics of phase transitions in water-Xe system. J. Chem. Phys. 2014; 141(3):034503. doi: https://doi.org/10.1063/1.4887069
Khudyakov АN, Polezhaeva TV, Zaitseva OO, Sergushkina MI, Solomina ON. Use of inert gases for the preservation of nuclear blood cells. Brazil. Arch. Biol. Technol. 2019; 62(42):19180204. doi: https://doi.org/10.1590/1678-4324-2019180204
Laptev DS, Polezhaeva TV, Zaitseva OO, Khudyakov AN, Solomina ON, Utemov SV. The use of inert gas xenon for cryopreservation of leukocytes. Bull. Exp. Biol. Med. 2014; 157(2):282-284. doi: https://doi.org/10.1007/s10517-014-2545-6
Sheleg S, Hixon H, Cohen B, Lowry D, Nedzved M. Cardiac Mitochondrial membrane stability after deep hypothermia using a xenon clathrate cryostasis protocol — an electron microscopy study. Int. J. Clin. Exp. Pathol. 2008;1(5):440-447.
Shishova NR, Uteshev VK, Kaurova SA, Browne RK, Gakhova EN. Cryopreservation of hormonally induced sperm for the conservation of threatened amphibians with Rana temporaria as a model research species. Theriogenology. 2011;75(2):220- 232. doi: https://doi.org/10.1016/j.theriogenology.2010.08.008
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 5, pp. 567-571, May, 2021
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Kaurova, S.A., Shvirst, N.E., Shishova, N.V. et al. Influence of Xenon on Survival of Sperm of Common Frog Rana temporaria during Slow Freezing. Bull Exp Biol Med 171, 596–600 (2021). https://doi.org/10.1007/s10517-021-05276-3
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DOI: https://doi.org/10.1007/s10517-021-05276-3