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Combined Preconditioning Reduces the Negative Influence of Cerebral Ischemia on the Morphofunctional Condition of CNS

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the influence of combined preconditioning (compound pQ-4 and moderate hypoxia) on morphometrical parameters of neuronal populations in hippocampal fields CA1 and CA3 in rats after bilateral ligation of the common carotid artery. Preconditioning produced a neuroprotective effect, improved survival of pyramidal neurons in the early and delayed periods of modeled ischemia, prevented the formation of necrotic and apoptotic neurons and hyperactivation of microglia, and protected endotheliocytes. The positive influence of preconditioning factors on the morphometric parameters of the brain under ischemic conditions agrees with the results of behavioral tests (open field and elevated plus maze) that demonstrated increased locomotor activity and exploratory behavior of animals.

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References

  1. Berezneva EY, Aleksandrova VV. The morphological features of neuronal populations of nuclei in thalamus and hippocampus of the brain of a rat white. Uspekhi Sovremen. Estestvozn. 2015;(9-2):287-289. Russian.

  2. Bureš J, Burešová O, Huston JP. Techniques and Basic Experiments for the Study of Brain and Behavior. Moscow, 1991. Russian.

  3. Zarubina IV, Shabanov PD. Neuroprotective Effects of Peptides during Ischemic Preconditioning. Bull. Exp. Biol. Med. 2016;160(4):448-451. doi: https://doi.org/10.1007/s10517-016-3193-9

    Article  CAS  PubMed  Google Scholar 

  4. Zenko MYu, Rybnikova EA. Pharmacological preconditioning. Integrativ. Fiziol. 2020;1(1):32-39. Russian.

    Google Scholar 

  5. Novikov VE, Levchenkova OS, Klimkina EI, Kulagin KN. Potentiation of the hypoxic preconditioning effect by antihypoxants. Obzory Klin. Farmakol. Lek. Ter. 2019;17(1):37-44. Russian.

    Article  Google Scholar 

  6. Novikov VE, Levchenkova OS, Pozhilova EV. Pharmacological preconditioning: opportunities and prospects. Vestn. Smolensk. Gos. Med. Akad. 2020;19(2):36-49. Russian.

    Google Scholar 

  7. Khudoerkov RM, Samoilenkova NS, Gavrilova SA, Pirogov YuA, Koshelev VB. Preconditioning as the method of neuroprotection in modeling brain infarction. Annaly Klin. Eksp. Nevrol. 2009;3(2):26-30. Russian.

    Google Scholar 

  8. Campbell BCV, De Silva DA, Macleod MR, Coutts SB, Schwamm LH, Davis SM, Donnan GA. Ischaemic stroke. Nat. Rev. Dis. Primers. 2019;5(1):70. doi: https://doi.org/10.1038/s41572-019-0118-8

    Article  PubMed  Google Scholar 

  9. Esposito E, Desai R, Ji X, Lo EH. Pharmacologic pre- and postconditioning for stroke: basic mechanisms and translational opportunity. Brain Circ. 2015;1(1):104-113. doi: https://doi.org/10.4103/2394-8108.166380

    Article  Google Scholar 

  10. Hao YM, He DW, Gao Y, Fang LN, Zhang PP, Lu K, Lu RZ, Li C. Review cerebral ischemic tolerance and preconditioning: methods, mechanisms, clinical applications, and challenges. Med. Sci. Monit. 2021;27:e929389. doi: https://doi.org/10.12659/MSM.929389

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Levchenkova OS, Novikov VE, Parfenov EA, Kulagin KN. Neuroprotective effect of antioxidants and moderate hypoxia as combined preconditioning in cerebral ischemia. Bull. Exp. Biol. Med. 2016;162(2):211-214. doi: https://doi.org/10.1007/s10517-016-3578-9

    Article  CAS  PubMed  Google Scholar 

  12. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. Academic Press, 2007.

  13. Yang Y, Lu F, Zhuang L, Yang S, Kong Y, Tan W, Gong Z, Zhan S. Combined preconditioning with hypoxia and GYKI-52466 protects rats from cerebral ischemic injury by HIF-1α/eNOS pathway. Am. J. Transl. Res. 2017;9(12):5308-5319.

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, Smolensk State Medical University, Ministry of Health of the Russian Federation, Smolensk, Russia

    O. S. Levchenkova, V. E. Novikov & E. A. Parfenov

  2. Department of Pathological Anatomy, Smolensk State Medical University, Ministry of Health of the Russian Federation, Smolensk, Russia

    Yu. S. Korneva, A. E. Dorosevich & E. A. Parfenov

Authors
  1. O. S. Levchenkova
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  2. V. E. Novikov
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  3. Yu. S. Korneva
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  4. A. E. Dorosevich
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  5. E. A. Parfenov
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Corresponding author

Correspondence to O. S. Levchenkova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 507-512, April, 2021

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Levchenkova, O.S., Novikov, V.E., Korneva, Y.S. et al. Combined Preconditioning Reduces the Negative Influence of Cerebral Ischemia on the Morphofunctional Condition of CNS. Bull Exp Biol Med 171, 489–493 (2021). https://doi.org/10.1007/s10517-021-05257-6

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  • DOI: https://doi.org/10.1007/s10517-021-05257-6

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