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In-Vitro Assessment of Hapten-Induced Immune Regulation in Children with Asthenoneurotic Syndrome

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Bulletin of Experimental Biology and Medicine Aims and scope

The study employed in vitro assay to examine the peculiarities of immune status in children with functional disorders of the autonomic nervous system diagnosticated as asthenoneurotic syndrome. In contrast to control children without asthenoneurotic syndrome, the examined group was characterized by significantly (p<0.05) elevated hapten-specific immunological sensitization (indicated by anti-Al IgG), induction of inflammatory reactions (IL-1), activation of apoptosis (CD3+CD95+ and р53) observed against the background inhibition of adaptive immune response (CD3+, CD3+CD4+, CD3+CD8+, CD16+56+, and CD19+), as well as hyperexpression of glutamic acid, NO, and VEGF combined with deficiency of serotonin. In cultured immunocompetent cells derived from children with hapten-modified immune status, the combined application of cytokine stimulator IL-1 with hapten sensitizer aluminum or with endocrine stimulator cortisol significantly (p<0.05) up-regulated expression of IL-8 and IL-10, but down-regulated production of IL-17 in a dose-dependent manner.

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Authors and Affiliations

  1. Federal Research Center of Medical and Preventive Technologies to Control Population Health Risks, Perm’, Russia

    О. V. Dolgikh, N. A. Nikonoshina & M. A. Guselnikov

Authors
  1. О. V. Dolgikh
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  2. N. A. Nikonoshina
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  3. M. A. Guselnikov
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Correspondence to О. V. Dolgikh.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 592-595, May, 2020

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Dolgikh, О.V., Nikonoshina, N.A. & Guselnikov, M.A. In-Vitro Assessment of Hapten-Induced Immune Regulation in Children with Asthenoneurotic Syndrome. Bull Exp Biol Med 169, 661–664 (2020). https://doi.org/10.1007/s10517-020-04949-9

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  • DOI: https://doi.org/10.1007/s10517-020-04949-9

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