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Cytological Characteristics of a Heterogeneous Population of Hepatocellular Carcinoma-29 Cells after Injection of Lithium Carbonate in the Experiment

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Bulletin of Experimental Biology and Medicine Aims and scope

Five cytological types of hepatocellular carcinoma-29 (G-29) grown in the muscle tissue of the thigh of experimental animals were identified by transmission electron microscopy; 89% of these were poorly differentiated type I-III cells. Lithium in a concentration of 20 mM produced a damaging effect on poorly differentiated G-29 cells: the number of cells with zones of intracellular component destruction and volume density of these zones increased, while volume density of cisterns of endoplasmic reticulum decreased. These results suggest that lithium carbonate can cause destructive changes in the heterogeneous population of G-29 cells during in vivo tumor development.

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References

  1. Bgatova NP, Omel’yanchuk LV, Pozhidaeva AA, Semeshin VF, Lykov AP, Poveshchenko OV, Makarova OP, Rachkovskaya LN, Borodin YI, Konenkov VI. Morphological Criteria of Cell Differentiation Stages in Experimental Hepatocarcinoma and Evaluation of Antitumor Drug Efficiency. Bull. Exp. Biol. Med. 2015;160(1):109-114. doi: https://doi.org/10.1007/s10517-015-3110-7

    Article  CAS  PubMed  Google Scholar 

  2. Gavrilova YuS, Bgatova NP, Solovieva AO, Trifonova KE, Lykov AP, Borodin YuI, Konenkov VI. The target cells of different lithium forms in heterogeneous population hepatocarcinoma-29. Tsitologiya. 2016;58(3):186-191. Russian.

  3. Kaledin VI, Zhukova NA, Nikolin VP, Popova NA, Beliaev MD, Baginskaya NV, Litvinova EA, Tolstikova TG, Lushnikova EL, Semenov DE. Hepatocarcinoma-29, a metastasizing transplantable mouse tumor inducing cachexia. Bull. Exp. Biol. Med. 2009;148(6):903-908.

    Article  CAS  Google Scholar 

  4. Cassidy JW, Caldas C, Bruna A. Maintaining tumor heterogeneity in patient-derived tumor xenografts. Cancer Res. 2015;15(75):2963-2968.

    Article  Google Scholar 

  5. da Motta Girardi D, Correa TS, Crosara Teixeira M, Dos Santos Fernandes G. Hepatocellular carcinoma: review of targeted and immune therapies. J. Gastrointest. Cancer. 2018;49(3):227-236.

    Article  Google Scholar 

  6. Freland L, Beaulieu JM. Inhibition of GSK3 by lithium, from single molecules to signaling networks. Front. Mol. Neurosci. 2012;5. ID 14. doi: https://doi.org/10.3389/fnmol.2012.00014

  7. Galle PR. Treating hepatobiliary cancers: the oncology way. Dig. Dis. 2017;35(4):384-386.

    Article  Google Scholar 

  8. Hammoud GM, Ibdah JA. Are we getting closer to understanding intratumor heterogeneity in hepatocellular carcinoma? Hepatobiliary Surg. Nutr. 2016;5(2):188-190.

    PubMed  PubMed Central  Google Scholar 

  9. Jeng KS, Chang CF, Jeng WJ, Sheen IS, Jeng CJ. Heterogeneity of hepatocellular carcinoma contributes to cancer progression. Crit. Rev. Oncol. Hematol. 2015;94(3):337-347.

    Article  Google Scholar 

  10. Li L, Song H, Zhong L, Yang R, Yang XQ, Jiang KL, Liu BZ. Lithium chloride promotes apoptosis in human leukemia NB4 cells by inhibiting glycogen synthase kinase-3 beta. Int. J. Med. Sci. 2015;12(10):805-810.

    Article  CAS  Google Scholar 

  11. Li L, Wang H. Heterogeneity of liver cancer and personalized therapy. Cancer Lett. 2016;379(2):191-197.

    Article  CAS  Google Scholar 

  12. Nault JC, Galle PR, Marquardt JU. The role of molecular enrichment on future therapies in hepatocellular carcinoma. J. Hepatol. 2018;69(1):237-247.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology, Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    Yu. S. Taskaeva & N. P. Bgatova

  2. Novosibirsk National Research State University, Novosibirsk, Russia

    Yu. S. Taskaeva

Authors
  1. Yu. S. Taskaeva
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  2. N. P. Bgatova
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Correspondence to Yu. S. Taskaeva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 6, pp. 739-743, June, 2019

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Taskaeva, Y.S., Bgatova, N.P. Cytological Characteristics of a Heterogeneous Population of Hepatocellular Carcinoma-29 Cells after Injection of Lithium Carbonate in the Experiment. Bull Exp Biol Med 167, 779–783 (2019). https://doi.org/10.1007/s10517-019-04621-x

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  • DOI: https://doi.org/10.1007/s10517-019-04621-x

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