We studied the intensity of age-specific changes in the dermis (number and proliferative activity of fibroblasts) in mice with normal and experimentally changed level of thyroid hormones. Receptors of thyroid hormones, TR-α and TR-β, in mouse dermal fibroblasts were identified by immunohistochemical methods. The relative expression of Thra, Thrb, and Dio2 genes was assessed by real-time PCR analysis. From the second to fifth month of life, the number of fibroblasts in the connective tissue layer of mouse skin decreased by 42.3%. The number of fibroblasts in the dermis of 5-month-old mice treated with Thyrozol significantly decreases by 25.9% (p<0.05), and vice versa, in mice receiving thyroxin this parameter increased by 4.7% in comparison with the control (p>0.05). TR-α and TR-β were identified in dermal fibroblasts in all groups of mice. No differences in the content TR-α and Thra gene expression in 2- and 5-month-old mice of the control and experimental were revealed. TR-β content in dermal fibroblasts of 2-month-old animals was maximum and exceeded this value in 5-month-old control mice by 25%. The number of these receptors decreased by 33.3% in mice treated with Thyrozol and increased by 25% in animals receiving thyroxin injection in comparison with the control. Relative expression of Thrb gene significantly increased only in mice treated with thyroxin. Comparative analysis of the relative expression of Dio2 gene revealed no differences between the experimental and control groups. Changes in the level of thyroid hormones, content of TR-β, and relative Thrb gene expression contribute to agerelated shifts in the number and proliferative activity of mouse dermal fibroblasts.
Similar content being viewed by others
References
Artykbaeva GM. Role of type 1 and 2 deiodinases in thyroid metabolism (review). Probl. Endokrinol. 2016;62(2):46-52. Russian.
Gunin AG, Osipova AA, Samoĭlova AV, Vasil’eva OV, Petrov VV, Kostin DM. The role of NUMB in morphogenetic changes in the uterus under the action of estrogens and glucocorticoids. Probl. Reproduktol. 2009;15(3):15-20. Russian.
Bodó E, Kany B, Gáspár E, Knüver J, Kromminga A, Ramot Y, Bíró T, Tiede S, van Beek N, Poeggeler B, Meyer K.C, Wenzel BE, Paus R. Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones. Endocrinology. 2010;151(4):1633-1642.
Cao X, Kambe F, Moeller LC, Refetoff S, Seo H. Thyroid hormone induces rapid activation of Akt/protein kinase Bmammalian target of rapamycin-p70S6K cascade through phosphatidylinositol 3-kinase in human fibroblasts. Mol. Endocrinol. 2005;19(1):102-112.
Cianfarani F, Baldini E, Cavalli A, Marchioni E, Lembo L, Teson M, Persechino S, Zambruno G, Ulisse S, Odorisio T, D’Armiento M. TSH receptor and thyroid-specific gene expression in human skin. J. Invest. Dermatol. 2010;130(1):93-101.
Contreras-Jurado C, García-Serrano L, Martínez-Fernández M, Ruiz-Llorente L, Paramio JM, Aranda A. Impaired hair growth and wound healing in mice lacking thyroid hormone receptors. PLoS One. 2014;9(9):e108137. doi: https://doi.org/10.1371/journal.pone.0108137.
Contreras-Jurado C, Lorz C, García-Serrano L, Paramio JM, Aranda A. Thyroid hormone signaling controls hair follicle stem cell function. Mol. Biol. Cell. 2015;26(7):1263-1272.
Gereben B, Zavacki AM, Ribich S, Kim BW, Huang SA, Simonides WS, Zeöld A, Bianco AC. Cellular and molecular basis of deiodinase-regulated thyroid hormone signaling. Endocr. Rev. 2008;29(7):898-938.
Gunin AG, Petrov VV, Golubtzova NN, Vasilieva OV, Kornilova NK. Age-related changes in angiogenesis in human dermis. Exp. Gerontol. 2014;55:143-151.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402-408.
Slominski A, Wortsman J, Kohn L, Ain KB, Venkataraman GM, Pisarchik A, Chung JH, Giuliani C, Thornton M, Slugocki G, Tobin DJ. Expression of hypothalamic-pituitary-thyroid axis related genes in the human skin. J. Invest. Dermatol. 2002;119(6):1449-1455.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 12, pp. 767-772, December, 2018
Rights and permissions
About this article
Cite this article
Gunin, A.G., Golubtsova, N.N., Kravtsova, O.A. et al. Number, Proliferative Activity, and Expression of Thyroid Hormone Receptors in Dermal Fibroblasts in Mice with Changed Thyroid Status. Bull Exp Biol Med 166, 797–801 (2019). https://doi.org/10.1007/s10517-019-04443-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04443-x