We studied proliferative activity of multipotent mesenchymal stromal cells on collagen matrices containing platelets stabilized with silver nanoparticles. Dose-dependent stimulation of the growth of stromal cells without their structural damage was observed in the presence of stabilized platelets in proportion of 20-120 mln per 105 multipotent mesenchymal stromal cells. The same doses of non-stabilized platelets produced less pronounced stimulation effect. In higher doses (≥180 mln) stabilized platelets inhibited cell proliferation and induced their damage. Stabilized platelets enhanced migration of multipotent mesenchymal stromal cells; after formation of the monolayer, they actively colonized deep layer of the collagen matrix. The formed monolayer of multipotent mesenchymal stromal cells survived over 14 days without appreciable cell damage.
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References
Vasilyev SA, Vinogradov VL, Karabudagova ZK. Platelet structure and functions. Gematol. Transfusiol. 2010;55(5):4-10. Russian.
Mazurov AV. Physiology and Pathology of Platelets. Moscow, 2011. Russian.
Makarov MS, Borovkova NV, Storozheva MV. Morphofunctional Properties of Human Platelets Treated with Silver nanoparticles. Bull. Exp. Biol. Med. 2017;164(2):241-246.
Makarov MS, Kobzeva EN, Vysochin IV, Borovkova NV, Khvatov VT Morphofunctional analysis of human platelets by vital staining. Bull. Exp. Biol. Med. 2014;156(3):409-412.
Makarov MS, Khavatov VB, Konyushko OI, Stoozheva MB, Borovkova NV, Ponomarev IN. The use of vital staining for evaluation of cell component of the biotransplant. Med. Alfavit. 2014;3(15):32-35. Russian.
Amable PR, Carias RB, Teixeira MV, da Cruz Pacheco I, Corrêa do Amaral RJ, Granjeiro JM, Borojevic R. Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors. Stem Cell Res. Ther. 2013;4(3):67. doi: https://doi.org/10.1186/scrt218.
Fagien S. Facial soft-tissue augmentation with injactable autologous and allogeneic human tissue collagen matrix (autologen and dermalogen). Plast. Reconstr. Surg. 2000;105(1):362-373.
Golebiewska EM, Poole AW. Secrets of platelet exocytosis — what do we really know about platelet secretion mechanisms. Br. J. Haematol. 2014;165(2):204-216.
Nurden AT, Nurden P, Sanchez M, Andia I, Anitua E. Platelets and wound healing. Front. Biosci. 2008;13:3532-3548.
Passaretti F, Tia M, D’Esposito V, De Pascale M, Del Corso M, Sepulveres R, Liguoro D, Valentino R, Beguinot F, Formisano P, Sammartino G. Growth-promoting action and growth factor release by different platelet derivatives. Platelets. 2014;25(4):252-256.
Platelets. Michelson AD, ed. Amsterdam, 2013.
Shrivastava S, Bera T, Singh SK, Singh G, Ramachandrarao P, Dash D. Characterization of antiplatelet properties of silver nanoparticles. ACS Nano. 2009;3(6):1357-1364.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 8, pp. 221-225, August, 2018
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Makarov, M.S., Storozheva, M.V., Borovkova, N.V. et al. Growth-Stimulating Effect of Human Platelets Stabilized with Silver Nanoparticles. Bull Exp Biol Med 166, 260–263 (2018). https://doi.org/10.1007/s10517-018-4328-y
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DOI: https://doi.org/10.1007/s10517-018-4328-y