We propose a cell model of the human small intestinal wall based on genetically modified Caco-2 cells that allows visualization and quantitative assessment of activation of NF-κB factor and related intracellular pathway by using fluorescence microscopy. A dose-dependent increase in fluorescence intensity of the obtained cells in response to TNFα exposure in concentrations of 1-100 ng/ml was demonstrated. It was found that this parameter correlates with a decrease in the transepithelial resistance of the cell monolayer in response to TNFα and can be used to assess the toxic effects of substances on epithelial cells of the human small intestine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rusanov AL, Luzgina NG, Lisitsa AV. Sodium Dodecyl Sulfate Cytotoxicity towards HaCaT Keratinocytes: Comparative Analysis of Methods for Evaluation of Cell Viability. Bull. Exp. Biol. Med. 2017;163(2):284-288.
Rusanov AL, Nakhod KV, Nakhod VI, Poverennaya EV, Petushkova NA, Luzgina NG. Changes in the Proteome of HaCaT Keratinocytes Induced by Cytotoxic Substance Triton X-100. Bull. Exp. Biol. Med. 2017;163(5):620-622.
Chen P, Migita S, Kanehira K, Sonezaki S, Taniguchi A. Development of sensor cells using NF-κB pathway activation for detection of nanoparticle-induced inflammation. Sensors (Basel). 2011;11(7):7219-7230.
Kim Y, Kim DM, Kim J.Y. Ginger extract suppresses inflammatory response and maintains barrier function in human colonic epithelial Caco-2 cells exposed to inflammatory mediators. J. Food Sci. 2017;82(5):1264-1270.
Ma TY, Iwamoto GK, Hoa NT, Akotia V, Pedram A, Boivin MA, Said HM. TNF-alpha-induced increase in intestinal epithelial tight junction permeability requires NF-kappa B activation. Am. J. Physiol. Gastrointest. Liver Physiol. 2004;286(3):G367-G376.
Marano CW, Lewis SA, Garulacan LA, Soler AP, Mullin JM. Tumor necrosis factor-α increases sodium and chloride conductance across the tight junction of Caco-2 BBE, a human intestinal epithelial cell line. J. Membr. Biol. 1998;161(3):263-274.
Oeckinghaus A, Ghosh S. The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb. Perspect. Biol. 2009;1(4):a000034. doi: https://doi.org/10.1101/cshperspect.a000034.
Rusanov AL, Luzgina NG, Barreto GE, Aliev G. Role of microfluidics in blood brain barrier permeability cell culture modeling: relevance to CNS disorders. CNS Neurol. Disord. Drug Targets. 2016;15(3):301-309.
Rusanov AL, Smirnova AV, Poromov AA, Fomicheva KA, Luzgina NG, Majouga AG. Effects of cadmium chloride on the functional state of human intestinal cells. Toxicol. In Vitro. 2015;29(5):1006-1011.
Sambuy Y, De Angelis I, Ranaldi G, Scarino M.L, Stammati A, Zucco F. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol. Toxicol. 2005;21(5):1-26.
Shen Y, Zhou M, Yan J, Gong Z, Xiao Y, Zhang C, Du P, Chen Y. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am. J. Physiol. Gastrointest. Liver Physiol. 2017;312(2):G123-G132.
Vila L, García-Rodríguez A, Cortés C, Marcos R, Hernández A. Assessing the effects of silver nanoparticles on monolayers of differentiated Caco-2 cells, as a model of intestinal barrier. Food Chem. Toxicol. 2018;116(Pt B):1-10.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 201-204, September, 2018
Rights and permissions
About this article
Cite this article
Rusanov, A.L., Luzgina, E.D., Vakhrushev, I.V. et al. A Cell Model of Human Small Intestinal Wall Based on Genetically Modified Caco-2 Cells. Bull Exp Biol Med 166, 174–177 (2018). https://doi.org/10.1007/s10517-018-4308-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4308-2