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Real-Time Recording of Platelet Adhesion to Fibrinogen-Coated Surface under Flow Conditions

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We present a testing system allowing real-time recording of the kinetics of platelet adhesion to fibrinogen-coated surface under flow conditions. The system consists of an optical flow chamber, semiconductor laser, two photodetectors, analog-to-digital converter, computer, and peristaltic pump. Platelet adhesion to fibrinogen-coated surface is recorded with two photodetectors and analyzed by the intensity of total internal reflection and scattered laser radiation at the boundary of the blood sample and fibrinogen-coated optical surface. Kinetics of platelet adhesion was studied as a function of shear rate and platelet concentration. The specificity of platelet adhesion with proteins on the surface of the flow chamber was verified by blocking IIb/IIIa glycoprotein complex on platelets with Fab2 fragments of monoclonal antibodies.

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Authors and Affiliations

  1. N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    Yu. N. Avtaeva

  2. Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia

    Yu. N. Avtaeva, I. S. Mel’nikov & Z. A. Gabbasov

Authors
  1. Yu. N. Avtaeva
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  2. I. S. Mel’nikov
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  3. Z. A. Gabbasov
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Correspondence to Yu. N. Avtaeva.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 48-52, January, 2018

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Avtaeva, Y.N., Mel’nikov, I.S. & Gabbasov, Z.A. Real-Time Recording of Platelet Adhesion to Fibrinogen-Coated Surface under Flow Conditions. Bull Exp Biol Med 165, 157–160 (2018). https://doi.org/10.1007/s10517-018-4119-5

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  • DOI: https://doi.org/10.1007/s10517-018-4119-5

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