We created an anisotropic material based on collagen sponge and reactive polylactide structured by laser photopolymerization. The combination of collagen with reactive polylactide improves the resistance of the formed matrices to biodegradation in comparison with collagen sponge, while the existence of sites with different mechanical characteristics and cell affinity on the matrix provides directed cell growth during their culturing. It was shown that reinforcement of the collagen sponges 7-fold increased the mean Young’s modulus for the hybrid matrix without affecting its cytotoxicity. The developed matrix provides cell adhesion and proliferation along reinforcement lines and can be used for fabrication of tissue engineering constructs.
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Glybochko PV, Alyaev YuG, Shekhter AB, Vinarov AZ, Istranov LP, Istranova EV, Aboyants RK, Lyundup AV, Krasheninnikov ME, Guller AE, Butnaru DV, Marisov LV, Kantimerov DF, Kurkov AV, Safronova EI, Vorob’eva EA, Iritsyan MM. PLGA mesh-collagen hybrid scaffold and tissue-engineered product in substitution urethroplasty: experimental validation. Urologiya. 2015;(6):5-13. Russian.
Timashev PS, Bardakova KN, Demina TS, Pudovkina GI, Novikov MM, Markov MA, Asyutin DS, Pimenova LF, Svidchenko EA, Ermakov AM, Selezneva II, Popov VK, Konovalov NA, Akopova TA, Solov’eva AB, Panchenko VYa, Bagrarashvili VN. A new biocompatible material based on solid phase modified chitosan for laser stereolithography. Sovremen. Tekhnol. Med. 2015;7(3):20-31. Russian.
Shekhter AB, Guller AE, Istranov LP, Istranova EV, Butnaru DV, Vinarov AZ, Zakharkina OL, Kurkov AV, Kantimerov DF, Antonov EN, Marisov LV, Glybochko PV. Morphology of collagen matrices for tissue engineering (biocompatibility, biodegradation, tissue response). Arkh. Patol. 2015;77(6):29-38. Russian.
Cao X, Wang J, Liu M, Chen Y, Cao Y, Yu X. Chitosan-collagen/organomontmorillonite scaffold for bone tissue engineering. Front. Mater. Sci. 2015;9(4):405-412.
Chan EC, Kuo SM, Kong AM, Morrison WA, Dusting GJ, Mitchell GM, Lim SY, Liu GS. Three dimensional collagen scaffold promotes intrinsic vascularisation for tissue engineering applications. PLoS One. 2016;11(2):e0149799. doi: https://doi.org/10.1371/journal.pone.0149799.
Dong C, Lv Y. Application of сollagen scaffold in tissue engineering: recent advances and new perspectives. Polymers. 2016;8(2):42. doi: https://doi.org/10.3390/polym8020042.
Grover CN, Cameron RE, Best SM. Investigating the morphological, mechanical and degradation properties of scaffolds comprising collagen, gelatin and elastin for use in soft tissue engineering. J. Mech. Behav. Biomed. Mater. 2012;10:62-74.
Kane RJ, Roeder RK. Effects of hydroxyapatite reinforcement on the architecture and mechanical properties of freeze-dried collagen scaffolds. J. Mech. Behav. Biomed. Mater. 2012;7:41-49.
Lee H, Ahn SH, Kim GH. Three-dimensional collagen/alginate hybrid scaffolds functionalized with a drug delivery system (DDS) for bone tissue regeneration. Chem. Mater. 2011;24(5):881-891.
Liu X, Huang C, Feng Y, Liang J, Fan Y, Gu Z, Zhang X. Reinforcement of a porous collagen scaffold with surface-activated PLA fibers. J. Biomater. Sci. Polym. Ed. 2010;21(6-7):963-977.
Lu H, Oh HH, Kawazoe N, Yamagishi K, Chen G. PLLAcollagen and PLLA-gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering. Sci. Technol. Adv. Mater. 2012;13(6). ID 064210.
Timashev P, Kuznetsova D, Koroleva A, Prodanets N, Deiwick A, Piskun Y, Bardakova K, Dzhoyashvili N, Kostjuk S, Zagaynova E, Rochev Y, Chichkov B, Bagratashvili V. Novel biodegradable star-shaped polylactide scaffolds for bone regeneration fabricated by two-photon polymerization. Nanomedicine (Lond). 2016;11(9):1041-1053.
Velasco MA, Narváez-Tovar CA, Garzón-Alvarado DA. Design, materials, and mechanobiology of biodegradable scaffolds for bone tissue engineering. Biomed. Res. Int. 2015;2015. ID 729076. doi: https://doi.org/10.1155/2015/729076.
Vilay V, Todo M. Tensile deformation behavior of collagen/PCL hybrid scaffolds for ligament tissue engineering. J. Solid Mech. Mater. Eng. 2013;7(3):463-472.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 31-37, January, 2018
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Bardakova, K.N., Grebenik, E.A., Istranova, E.V. et al. Reinforced Hybrid Collagen Sponges for Tissue Engineering. Bull Exp Biol Med 165, 142–147 (2018). https://doi.org/10.1007/s10517-018-4116-8
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DOI: https://doi.org/10.1007/s10517-018-4116-8