The effects of a non-selective blocker of NO synthases LNNA in a dose of 25 mg/kg and nitrates KNO3, NaNO3, Mg(NO3)2, and Ca(NO3)2 in doses of 5 and 50 mg/kg were studied on the model of experimental ischemic stroke induced by bilateral occlusion of the common carotid arteries. Wistar rats were randomized into 40 treatment (n=960) and 8 control (n=192) groups. Treatment group rats received injection of either nitrate, or LNNA, or a combination LNNA+nitrate. All substances were administered intraperitoneally 1 h prior to brain ischemia or 5 sec after occlusion of the common carotid arteries. Control rats received the same volume of 0.9% NaCl at the same terms. The non-selective inhibitor of NO synthases LNNA administered against the background of brain ischemia increased neurological deficit and animal mortality. Nitrate/nitrite reductase system had protective properties depending on the cation of nitrate and concentration of a nitrate during inhibition of NO synthase system under conditions of brain ischemia and hypoxia.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 1, pp. 38-42, January, 2018
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Kuzenkov, V.S. Protective Role of Nitrate/Nitrite Reductase System during Transient Global Cerebral Ischemia. Bull Exp Biol Med 165, 31–35 (2018). https://doi.org/10.1007/s10517-018-4092-z
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DOI: https://doi.org/10.1007/s10517-018-4092-z