We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 207-212, October, 2017
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Voronova, А.D., Stepanova, O.V., Valikhov, M.P. et al. Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries. Bull Exp Biol Med 164, 523–527 (2018). https://doi.org/10.1007/s10517-018-4025-x
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DOI: https://doi.org/10.1007/s10517-018-4025-x