Skip to main content

Advertisement

SpringerLink
Log in

Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells

  • MORPHOLOGY AND PATHOMORPHOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients’ histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nizyaeva NV, Volkova YS, Mullabaeva SM, Shchegolev AI. The methodical bases for placental tissue examination and the optimization of material pre-preparation regimens. Akush. Gin. 2014;(8):10-18. Russian.

  2. Rylova YV, Milovanova NV, Gordeeva MN, Savilova AM. Characteristics of multipotent mesenchymal stromal cells from human terminal placenta. Bull. Exp. Biol. Med. 2015; 159(2):253-257.

    Article  CAS  PubMed  Google Scholar 

  3. Savilova AM, Zakharov AV, Metlyuk EA, Serdyuk YV, Veryasov VN, Krasnyi AM, Trofimov DY. Comparison of the expression of immunomodulatory factors in cultures of mesenchymal stromal cells from human extraembryonic tissues. Bull. Exp. Biol. Med. 2014;158(4):555-560.

    Article  Google Scholar 

  4. Shchegolev AI, Dubova EA, Pavlov KA. Placenta Morphology. Moscow, 2010. Russian.

  5. Benirschke K, Burton GJ, Baergen RN. Pathology of the Human Placenta. 6th ed. New York, 2012.

  6. Burton GJ, Sebire NJ, Myatt L, Tannetta D, Wang YL, Sadovsky Y, Staff AC, Redman CW. Optimising sample collection for placental research. Placenta. 2014;35(1):9-22.

    Article  CAS  PubMed  Google Scholar 

  7. Hwang JH, Shim SS, Seok OS, Lee HY, Woo SK, Kim BH, Song HR, Lee JK, Park YK. Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow. J. Korean. Med. Sci. 2009;24(4):547-554.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Lindenmair A, Hatlapatka T, Kollwig G, Hennerbichler S, Gabriel C, Wolbank S, Redl H, Kasper C. Mesenchymal stem or stromal cells from amnion and umbilical cord tissue and their potential for clinical applications. Cells. 2012;1(4):1061-1088.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Male V, Gardner L, Moffett A. Isolation of cells from the feto-maternal interface. Curr. Protoc. Immunol. 2012. Chapter 7:Unit 7.40.1-11. doi: 10.1002/0471142735.im0740s97.

  10. Mathews S, Lakshmi Rao K, Suma Prasad K, Kanakavalli MK, Govardhana Reddy A, Avinash Raj T, Thangaraj K, Pande G. Propagation of pure fetal and maternal mesenchymal stromal cells from terminal chorionic villi of human term placenta. Sci. Rep. 2015;5:10054. doi: 10.1038/srep10054.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Mendez-Figueroa H, Chien EK, Ji H, Nesbitt NL, Bharathi SS, Goetzman E. Effects of labor on placental fatty acid b oxidation. J. Matern. Fetal Neonatal Med. 2013;26(2):150-154.

    Article  CAS  PubMed  Google Scholar 

  12. Queiros da Mota V, Prodhom G, Yan P, Hohlfheld P, Greub G, Rouleau C. Correlation between placental bacterial culture results and histological chorioamnionitis: a prospective study on 376 placentas. J. Clin. Pathol. 2013;66(3):243-248.

    Article  PubMed  Google Scholar 

  13. Redline RW. Villitis of unknown etiology: noninfectious chronic villitis in the placenta. Hum. Pathol. 2007;38(10):1439-1446.

    Article  PubMed  Google Scholar 

  14. Sukhikh GT, Malaitsev VV, Bogdanova IM. Application potential of human fetal stem/progenitor cells in cell therapy. Bull. Exp. Biol. Med. 2008;145(1):114-121.

    Article  CAS  PubMed  Google Scholar 

  15. Veryasov VN, Savilova AM, Buyanovskaya OA, Chulkina MM, Pavlovich SV, Sukhikh GT. Isolation of mesenchymal stromal cells from extraembryonic tissues and their characteristics. Bull. Exp. Biol. Med. 2014. Vol. 157(1):119-124.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia

    N. V. Nizyaeva, M. N. Nagovitsyna, G. V. Kulikova, U. N. Tumanova, R. A. Poltavtseva, I. A. Fedorova, M. N. Yushina, S. V. Pavlovich & A. I. Shchyogolev

Authors
  1. N. V. Nizyaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. N. Nagovitsyna
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. V. Kulikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. U. N. Tumanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. A. Poltavtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. A. Fedorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. N. Yushina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S. V. Pavlovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. I. Shchyogolev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. I. Shchyogolev.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 10, pp. 500-506, October, 2016

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nizyaeva, N.V., Nagovitsyna, M.N., Kulikova, G.V. et al. Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells. Bull Exp Biol Med 162, 501–506 (2017). https://doi.org/10.1007/s10517-017-3648-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3648-7

Key Words

Search

Navigation