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Isolation of Mesenchymal Stromal Cells from Extraembryonic Tissues and Their Characteristics

  • Cell Technologies in Biology and Medicine (Translated from Kletochnye Tekhnologii v Biologii i Meditsine)
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Bulletin of Experimental Biology and Medicine Aims and scope

We describe a method of isolation of human mesenchymal stromal cells from the umbilical cord (Wharton’s jelly) and human placenta: amnion, placental villi, and trophoblast. Morphology, immunophenotypic characteristics, and differentiation potencies of isolated cells were studied. The capacity of mesenchymal stromal cells from extraembryonic tissues to osteogenic, adipogenic, and chondrogenic differentiation was demonstrated and the dynamics of this process was described. The isolated cells met the criteria for multipotent mesenchymal stem cells.

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Authors and Affiliations

  1. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    V. N. Veryasov, A. M. Savilova, O. A. Buyanovskaya, M. M. Chulkina, S. V. Pavlovich & G. T. Sukhikh

Authors
  1. V. N. Veryasov
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  2. A. M. Savilova
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  3. O. A. Buyanovskaya
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  4. M. M. Chulkina
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  5. S. V. Pavlovich
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  6. G. T. Sukhikh
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Corresponding author

Correspondence to A. M. Savilova.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 3-9, January, 2014

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Veryasov, V.N., Savilova, A.M., Buyanovskaya, O.A. et al. Isolation of Mesenchymal Stromal Cells from Extraembryonic Tissues and Their Characteristics. Bull Exp Biol Med 157, 119–124 (2014). https://doi.org/10.1007/s10517-014-2506-0

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  • DOI: https://doi.org/10.1007/s10517-014-2506-0

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