Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7+ epithelial cells that disappeared by passage 2. The cultures from the endometrium could be induced to adipogenic, osteogenic and chondrogenic differentiation in vitro. These findings suggest that human endometrium is a convenient source of biomaterial for minimally invasive isolation of cultures that exhibit typical morphology and immunophenotypic profile of resident multipotent mesenchymal stromal cells retain high viability in vitro.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 252-257, October, 2015
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Savilova, A.M., Yushina, M.N., Rudimova, Y.V. et al. Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium. Bull Exp Biol Med 160, 560–564 (2016). https://doi.org/10.1007/s10517-016-3218-4
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DOI: https://doi.org/10.1007/s10517-016-3218-4