We studied the mechanisms of adaptation of human breast cancer cells MCF-7 to hypoxia and analyzed the role of AMPK/mTOR signaling pathway in the maintenance of cell proliferation under hypoxic conditions. It was found that long-term culturing (30 days or more) of MCF-7 cells under hypoxic conditions induced their partial adaptation to hypoxia. Cell adaptation to hypoxia was associated with attenuation of hypoxia-dependent AMPK induction with simultaneous constitutive activation of mTOR and Akt. These findings suggest that these proteins can be promising targets for targeted therapy of tumors developing under hypoxic conditions.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 246-251, October, 2015
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Sorokin, D.V., Scherbakov, A.M., Yakushina, I.A. et al. The Mechanism of Adaptation of Breast Cancer Cells to Hypoxia: Role of AMPK/mTOR Signaling Pathway. Bull Exp Biol Med 160, 555–559 (2016). https://doi.org/10.1007/s10517-016-3217-5
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DOI: https://doi.org/10.1007/s10517-016-3217-5