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Cell-Mediated Hemolytic Activity of Nosocomial Pseudomonas Aeruginosa Strains

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Cell-mediated hemolysis and adhesion index of nosocomial P. aeruginosa strains were experimentally studied. The highest hemoglobin release was recorded after centrifugation of erythrocyte and bacterial cell suspension preincubated at 37 C. All cultures were referred to highly adherent variants. The relationship between P. aeruginosa adhesion activity and erythrocyte lysis was found only in “passive” cell–cell contact. No correlation between cellassociated hemolysis and hemolysis caused by secreted factors was detected. It seems that the cytotoxicity of the studied P. aeruginosa strains was determined by ExoU and ExoS third type secretion effectors.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, Perm, Russia

    M. V. Kuznetsova

  2. E. A. Wagner Perm State Medical Academy, Ministry of Health of the Russian Federation, Perm, Russia

    M. V. Kuznetsova & T. I. Karpunina

Authors
  1. M. V. Kuznetsova
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  2. T. I. Karpunina
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Correspondence to M. V. Kuznetsova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 2, pp. 219-223, February, 2015

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Kuznetsova, M.V., Karpunina, T.I. Cell-Mediated Hemolytic Activity of Nosocomial Pseudomonas Aeruginosa Strains. Bull Exp Biol Med 159, 258–261 (2015). https://doi.org/10.1007/s10517-015-2936-3

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  • DOI: https://doi.org/10.1007/s10517-015-2936-3

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