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Statistic Parametric Mapping of Changes in Gene Activity in Animal Brain during Acoustic Stimulation

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We analyzed the expression of transcription factor c-Fos induced by neural activity in the mouse brain after acoustic stimulation. The brain sections of the animals subjected to acoustic stimulation and controls were immunohistochemically stained for c-Fos protein. Statistical parametric mapping (SPM) was used to identify group differences in the acquired images. c-Fos expression was significantly higher in the auditory cortex, amygdala, and hippocampus CA3 area after tone presentation. The proposed combination of SPM with molecularbiological approach to visualization of transcription in the nerve cells makes it possible to identify the collaborative activation of distant brain structures assumed to be the components of united functional systems.

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References

  1. A. B. Abramova, K. V. Anokhin, O. N. Dolgov, et al., Vestn. Nov. Med. Tekhn., 1, 47–53 (1997).

  2. K. V. Anokhin, Zh. Vyssh. Nervn. Deyat., 47, No. 2, 261–279 (1997).

    CAS  Google Scholar 

  3. D. N. Barry and S. Commins, Rev. Neurosci., 222, No. 2, 131–142 (2011).

    Google Scholar 

  4. K. J. Friston, J. Ashburner, S. J. Kiebel, et al., Statistical Parametric Mapping: The Analysis of Functional Brain Images, London (2007).

  5. J. F. Guzowski, J. A. Timlin, B. Roysam, et al., Curr. Opin. Neurobiol., 15, No. 5, 599–606 (2005).

    Article  PubMed  CAS  Google Scholar 

  6. J. Hall, K. L. Thomas, and B. J. Everitt, Eur. J. Neurosci., 13, No. 7, 1453–1458 (2001).

    Article  PubMed  CAS  Google Scholar 

  7. E. S. Lein, M. J. Hawrylycz, N. Ao, et al., Nature, 445, 168–176 (2007).

    Article  PubMed  CAS  Google Scholar 

  8. S. Maren, Eur. J. Neurosci., 28, No. 8, 1661–1666 (2008).

    Article  PubMed  Google Scholar 

  9. Milanovic S., Radulovic J., Laban O., et al., Brain Res., 784, Nos. 1–2, 37–47 (1998).

    Article  PubMed  CAS  Google Scholar 

  10. A. Osokin, D. Vetrov, A. Lebedev, et al., Computational Intelligence Methods for Bioinformatics and Biostatistics – 7th International Meeting (2010) pp. 86–97.

  11. M. Peter, H. Scheuch, T. R. Burkard, et al., Genes. Brain Behav., 11, No. 3, 314–324 (2012).

    Article  PubMed  CAS  Google Scholar 

  12. T. D. Poeppel and B. J. Krause, Methods, 44, No. 4, 315–328 (2008).

    Article  PubMed  CAS  Google Scholar 

  13. J. Radulovic, J. Kammermeier, and J. Spiess, J. Neurosci., 18, No. 18, 7452–7461 (1998).

    PubMed  CAS  Google Scholar 

  14. M. Wada, N. Higo, S. Moizumi, and S. Kitazawa, PLoS ONE, 5, No. 5, e10483 (2010).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia

    E. M. Amelchenko, S. V. Zworikina, S. A. Chekhov & K. V. Anokhin

  2. Department of Neurosciences, NBIC Center, Kurchatov Institute National Research Center, Moscow, Russia

    E. M. Amelchenko, A. A. Osokin, S. V. Zworikina, S. A. Chekhov, P. A. Voronin, D. P. Vetrov & K. V. Anokhin

  3. Faculty of Computational Mathematics and Cybernetics, Moscow, Russia

    A. A. Osokin & D. P. Vetrov

  4. Mechanics and Mathematics Faculty of M. V. Lomonosov Moscow State University, Moscow, Russia

    A. E. Lebedev & V. V. Galatenko

Authors
  1. E. M. Amelchenko
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  2. A. A. Osokin
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  3. S. V. Zworikina
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  4. S. A. Chekhov
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  5. A. E. Lebedev
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  6. P. A. Voronin
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  7. V. V. Galatenko
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  8. D. P. Vetrov
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  9. K. V. Anokhin
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Corresponding author

Correspondence to E. M. Amelchenko.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 10, pp. 657–660, November, 2012.

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Amelchenko, E.M., Osokin, A.A., Zworikina, S.V. et al. Statistic Parametric Mapping of Changes in Gene Activity in Animal Brain during Acoustic Stimulation. Bull Exp Biol Med 154, 697–699 (2013). https://doi.org/10.1007/s10517-013-2032-5

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  • DOI: https://doi.org/10.1007/s10517-013-2032-5

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