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Cell Composition of the Primary Culture of Fetal Liver

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Bulletin of Experimental Biology and Medicine Aims and scope

Fetal liver is known as a source of multipotent mesenchymal stromal cells. These cells are routinely isolated by adhesion to plastic, but thus prepared culture is contaminated by other cells. For instance, primary cell culture of from rat fetal liver, apart from fibroblasts with phenotypic characteristics of mesenchymal stromal cells, contained skeletal muscle precursors, myofibroblasts, and epitheliocytes expressing cytokeratin-19 (the latter was also detected in some fibroblast-like cells probably undergoing epithelio-mesenchymal transition). During passaging, fibroblasts become practically the only cell type in the culture.

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Authors and Affiliations

  1. N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    O. V. Payushina, N. N. Butorina, O. N. Sheveleva, M. N. Kozhevnikova & V. I. Starostin

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  1. O. V. Payushina
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  2. N. N. Butorina
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  3. O. N. Sheveleva
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  4. M. N. Kozhevnikova
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  5. V. I. Starostin
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Correspondence to O. V. Payushina.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 224-232, October, 2012

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Payushina, O.V., Butorina, N.N., Sheveleva, O.N. et al. Cell Composition of the Primary Culture of Fetal Liver. Bull Exp Biol Med 154, 566–573 (2013). https://doi.org/10.1007/s10517-013-2001-z

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  • DOI: https://doi.org/10.1007/s10517-013-2001-z

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