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Multidirectional Effects of Calmodulin Kinase II on Transmitter Release in Mature and Newly Formed Mouse Motor Synapses

  • BIOPHYSICS AND BIOCHEMISTRY
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Bulletin of Experimental Biology and Medicine Aims and scope

Calmodulin inhibitor W-7 did not cause changes in the quantal content of postsynaptic endplate potentials (EPP) in newly formed synapses, but prevented facilitation of acetylcholine secretion induced by L-type Ca2+ channels blocker nitrendipine. CaMKII inhibitor KN-62 produced similar effect and suppressed the increase in EPP quantal content caused by blockade of L-type Ca2+ channels. Phosphatase PP2A inhibitor okadaic acid significantly facilitated secretion in newly formed synapses; the effect was completely blocked by KN-62. In mature synapses, okadaic acid had no effect on transmitter secretion. KN-62 increased EPP quantal content. We hypothesize that CaMKII produced different effects on acetylcholine secretion in mature and immature synapses depending on specificity of calcium signaling and PP2A phosphatase activity.

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References

  1. O. P. Balezina, P. O. Bogatcheva, and T. Yu. Orlova, Byull. Eksper. Biol., 143, No. 2, 128-132 (2007).

    Google Scholar 

  2. P. O. Bogatcheva, E. V. Ezhova, and O. P. Balezina, Ibid., 149, No. 2, 127-132 (2010).

    Google Scholar 

  3. F. S. Gorelick, J. K. Wang, and Y. Lai, J. Biol. Chem., 263, No. 33, 17,209-17,212 (1988).

    CAS  Google Scholar 

  4. M. Graupner and N. Brunel, PLoS. Comput. Biol., 3, No. 11, e221 (2007).

    Article  PubMed  Google Scholar 

  5. H. L. Hinds, I. Goussakov, K. Nakarawa, et al., Proc. Natl. Acad. Sci. USA, 100, No. 7, 4275-4280 (2003).

    Article  PubMed  CAS  Google Scholar 

  6. M. Igarashi and M. Watanabe, Neurosci. Res., 58, No. 3, 226-233 (2007).

    Article  PubMed  CAS  Google Scholar 

  7. Q. Liu, B. Chen, Q. Ge, and Z. W. Wang, J. Neurosci., 27, No. 39, 10,404-10,413 (2007).

    Article  CAS  Google Scholar 

  8. A. Menegon, C. Verderio, and C. Leoni, J. Neurosci., 22, No. 16, 7016-7026 (2002).

    PubMed  CAS  Google Scholar 

  9. M. A. Mukhamedyarov, J. O. Kochunova, E. R. Yusupova, et al., Brain Res. Bull., 81, No. 6, 613-616 (2010).

    Article  PubMed  CAS  Google Scholar 

  10. J. J. Saucerman and D. M. Bers, Biophys. J., 95, No. 10, 4597-4612 (2008).

    Article  PubMed  CAS  Google Scholar 

  11. S. I. Walaas, F. S. Gorelick, and P. Greengard, Synapse, 3, No. 4, 356-362 (1989).

    Article  PubMed  CAS  Google Scholar 

  12. Z. W. Wang, Mol. Neurobiol., 38, No. 2, 153-166 (2008).

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia

    P. O. Bogatcheva & O. P. Balezina

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  1. P. O. Bogatcheva
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  2. O. P. Balezina
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Correspondence to P. O. Bogatcheva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 9, pp. 294-298, September, 2012

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Bogatcheva, P.O., Balezina, O.P. Multidirectional Effects of Calmodulin Kinase II on Transmitter Release in Mature and Newly Formed Mouse Motor Synapses. Bull Exp Biol Med 154, 316–319 (2013). https://doi.org/10.1007/s10517-013-1940-8

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  • DOI: https://doi.org/10.1007/s10517-013-1940-8

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