The intracellular levels of superoxide \( {\text{O}}_2^{-} \) and nitric oxide NO were directly measured under intravital conditions in cultured human endotheliocytes ECV 304 and carcinoma cells HeLa G-63. Comparative analysis of changes in the intracellular levels of superoxide and NO induced by ascorbic acid revealed a negative correlation between NO and \( {\text{O}}_2^{-} \) levels, whose strength depended on concentration of the acid. In pharmacological concentrations, ascorbic acid induced apoptotic death of carcinoma cells, but did not trigger apoptosis of endotheliocytes. The study demonstrated a possible way of cytotoxic action of ascorbic acid in pharmacological concentrations.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 150, No. 11, pp. 577–581, November, 2010
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Giliano, N.Y., Konevega, L.V. & Noskin, L.A. Modification of Intracellular Level of Free Radicals and Apoptosis in Cultured Human Endotheliocytes and Carcinoma Cells. Bull Exp Biol Med 150, 645–648 (2011). https://doi.org/10.1007/s10517-011-1212-4
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DOI: https://doi.org/10.1007/s10517-011-1212-4