Plasma γ-globulin fraction proteins, copper and zinc cations, and metal complexes formed by these cations and human serum γ-globulin induce blast transformation of splenocytes from BALB/c mice at a level comparable to that induced by concanavalin A. Zinc bound to γ-globulin reduces by 25% and copper in complex with this protein stimulates by 1.6 times its capacity to induce blast transformation. Combinations with concanavalin A reproduce the effects of γ-globulin–metal complex under conditions of mitogen induction. Incorporation of 3H-thymidine in splenocytes incubated with combinations of γ-globulin–copper metalcomplex, copper cations, and control protein with concanavalin A was by 1.4, 1.3 (p<0.1), and 1.25 times higher (p<0.05), respectively, than after incubation with concanavalin A alone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. Ya. Kulberg, Yu. B. Berkun, and S. B. Cheknev, Immunologiya, No. 6, 7–9 (1997).
S. B. Cheknev, Ya. G. Ashmanova, E. E. Babaeva, et al., Byull. Eksp. Biol. Med., 118, No. 12, 625–630 (1994).
S. B. Cheknev and E. E. Babaeva, Med. Immunol., 6, Nos. 3–5, 186–189 (2004).
S. B. Cheknev, A. A. Babaynts, and E. A. Denisova, Byull. Eksp. Biol. Med., 146, No. 11, 526–530 (2008).
S. B. Cheknev, A. A. Babaynts, I. E. Efremova, and E. N. Yushkovets, Ibid., 147, No. 5, 544–548 (2009).
S. B. Cheknev, I. E. Efremova, E. A. Denisova, and E. N. Yushkovets, Ros. Immunol. Zh., 2, No. 1, 55–62 (2008).
E. N. Yushkovets, I. E. Efremova, A. A. Babaynats, and S. B. Cheknev, Ibid., 4, No. 1, 41–47 (2010).
S. Bala, M. L. Failla, and J. K. Lunney, J. Nutr., 121, No. 5, 745–753 (1991).
B. Bao, A. S. Prasad, F. W. J. Beck, and M. Goldmere, Am. J. Physiol. Endocrinol. Metab., 285, No. 5, 1095–1102 (2003).
U. Carpentieri, J. Myers, C. W. Daeschner 3rd, and M. E. Haggard, Biol. Trace Elem. Res., 16, No. 2, 165–176 (1988).
R. G. Hopkins and M. L. Failla, J. Nut., 129, No. 3, 596–601 (1999).
S. S. Percival, Am. J. Clin. Nutr., 67, No. 5, Suppl., 1064–1068 (1998).
L. Rink and H. Kirchner, J. Nutr., 130, 5S Suppl., 1407S-1411S (2000).
G. L. Warner and D. A. Lawrence, Int. J. Immunopharmacol., 10, No. 5, 629–637 (1988).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 150, No. 11, pp. 555–558, November, 2010
Rights and permissions
About this article
Cite this article
Cheknev, S.B., Grigorjeva, E.A., Nikolaeva, T.N. et al. Mouse Splenocyte Blast Transformation in the Presence of Plasma γ-Globulin Fraction Proteins and Their Complexes with Copper and Zinc. Bull Exp Biol Med 150, 624–626 (2011). https://doi.org/10.1007/s10517-011-1206-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-011-1206-2