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Immunohistochemical Study for the Expression of Bcl-2 Family Proteins in Walker 256 Carcinosarcoma Cells under the Influence of Cytostatic Drugs

  • Morphology and Pathomorphology
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Bulletin of Experimental Biology and Medicine Aims and scope

Immunohistochemical study was performed to evaluate the expression of Bcl-2 family proteins (Bcl-2, Bax, and Bad) in Walker 256 carcinosarcoma cells after implantation into the thigh muscle of male Wistar rats (106 cells). The experiment was conducted under conditions of spontaneous tumor development and individual or combined treatment with melatonin and cyclophosphamide. The use of melatonin as monotherapy or in combination with cyclophosphamide was followed by a signifi cant decrease in Bcl-2 expression in carcinosarcoma cells. The Bcl-2/Bax and Bcl-2/Bad ratio was signifi cantly reduced under these conditions (particularly after combined treatment with cytostatic drugs). These changes were accompanied by a signifi cant (by 93.61%) decrease in the volume of transplantable tumor on day 14. Daily treatment with melatonin was accompanied by signifi cant changes in the structure of Walker 256 carcinosarcoma. It was manifested in the formation of connective tissue septa and pseudofollicles.

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Authors and Affiliations

  1. Novosibirsk State Medical University, Federal Agency for Health Care and Social Development, Novosibirsk, Russia

    E. V. Ovsjanko, S. A. Arkhipov, A. V. Efremov & Ya. U. Ovsjanko

  2. Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia

    E. L. Lushnikova & L. M. Nepomnyashchikh

  3. E. N. Meshalkin Novosibirsk Institute for Pathology of Blood Circulation, Russian Medical Technologies, Novosibirsk, Russia

    P. M. Larionov

Authors
  1. E. V. Ovsjanko
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  2. E. L. Lushnikova
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  3. P. M. Larionov
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  4. S. A. Arkhipov
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  5. L. M. Nepomnyashchikh
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  6. A. V. Efremov
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  7. Ya. U. Ovsjanko
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Correspondence to E. V. Ovsjanko.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 148, No. 10, pp. 455-460, October, 2009

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Ovsjanko, E.V., Lushnikova, E.L., Larionov, P.M. et al. Immunohistochemical Study for the Expression of Bcl-2 Family Proteins in Walker 256 Carcinosarcoma Cells under the Influence of Cytostatic Drugs. Bull Exp Biol Med 148, 650–655 (2009). https://doi.org/10.1007/s10517-010-0787-5

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  • DOI: https://doi.org/10.1007/s10517-010-0787-5

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