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Use of Oligonucleotides Conjugated to Gold Nanoparticles and Streptavidin for Amplification of Optical Biosensor Signal during Detection of Telomeric Repeats

  • Nanotechnology
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Bulletin of Experimental Biology and Medicine Aims and scope

Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied by the surface plasmon resonance method. Conjugation of the probe with streptavidin and gold nanoparticles was shown to amplify the signal at similar concentrations of this probe (by 60 and 300 times, respectively). Nanoparticles can be used for biosensor signal amplification in studying the telomerase activity of malignant cells.

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Authors and Affiliations

  1. V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia

    S. P. Rad’ko, S. A. Voronina, A. V. Gromov, O. V. Gnedenko, N. V. Bodoev, A. S. Ivanov & K. N. Yarygin

Authors
  1. S. P. Rad’ko
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  2. S. A. Voronina
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  3. A. V. Gromov
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  4. O. V. Gnedenko
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  5. N. V. Bodoev
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  6. A. S. Ivanov
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  7. K. N. Yarygin
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Corresponding author

Correspondence to K. N. Yarygin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 6, pp. 689-692, June, 2009

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Rad’ko, S.P., Voronina, S.A., Gromov, A.V. et al. Use of Oligonucleotides Conjugated to Gold Nanoparticles and Streptavidin for Amplification of Optical Biosensor Signal during Detection of Telomeric Repeats. Bull Exp Biol Med 147, 746–749 (2009). https://doi.org/10.1007/s10517-009-0616-x

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  • DOI: https://doi.org/10.1007/s10517-009-0616-x

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