Abstract
Silver and gold nanoparticle-aptamer conjugates have been extensively utilized as biosensors and microscopic vehicles that deliver a therapeutic cargo to cells. Here, we describe facile procedures to attach nucleic acid aptamers with a free thiol group to silver or gold nanoparticles. Methods to purify the nanoparticle-aptamer conjugates, verify aptamer attachment, and quantify aptamer-nanoparticle ratios are also discussed and compared. Additionally, a simple protocol that describes the aqueous synthesis of gold nanoparticles (~10 nm) is included.
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Acknowledgments
This work was supported by funding from SC INBRE #5P20GM103499 and MADE in SC EPSCoR, NSF #1655740.
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Quarles, J.D., Livingston, A.T., Wood, A.E., Fernandez, T.G. (2023). Preparation of Nucleic Acid Aptamer Functionalized Silver/Gold Nanoparticle Conjugates Using Thiol-Substituted Oligonucleotides. In: Afonin, K.A. (eds) RNA Nanostructures. Methods in Molecular Biology, vol 2709. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3417-2_8
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DOI: https://doi.org/10.1007/978-1-0716-3417-2_8
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