We compared the effects of dextrans with a molecular weight of 35 kDa oxidized by chemical (ODch) and radiochemical (ODr) methods on oxidative and metabolic functions of peritoneal macrophages from BALB/c mice in vitro and in vivo. It was found that none type of dextrans exhibits chemiluminescent properties. In vitro study showed that ODch had a priming effect on mouse peritoneal macrophages, while ODr did not potentiate the oxidative and metabolic response of cells to zymosan. Being injected intraperitoneally, ODr more markedly enhanced chemiluminescent response of mouse peritoneal macrophages and reduced their viability than ODch. Thus, both dextrans are biocompatible, but in ODch this parameter is higher.
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References
E. D. Goldberg, A. M. Dygai, and V. P. Shakhov, Methods of Tissue Culture in Hematology [in Russian], Tomsk (1992).
N. K. Zenkov, V. Z. Lankin, and E. B. Men’shchikova, Oxidative Stress: Biochemical and Pathophysiological Aspects [in Russian], Moscow (2001).
D. N. Mayanskii, D. D. Tsyrendorzhiev, O. P. Makarova, et al., Diagnostic Value of Leukocytic Tests. Part 2. Evaluation of Biocydal Properties of Leukocytes (Methodical Recommendations) [in Russian], Novosibirsk (1996).
S. G. Panasenko, A. B. Pupyshev, Yu. N. Kurunov, and V. A. Shkurupiy, Method of Obtaining Isoniazid-Dextran Conjugate [in Russian], Patent RU No. 2125451 of 27.01.1999
V. A. Sharpatyi, Radiats. Biol. Radioekol., 39, No. 1, 156–161 (1999).
V. A. Shkurupiy, Yu. N. Kurunov, O. V. Grishin, et al., Method of Obtaining Prolonged-Action Isoniazid, Patent RU No. 2143900 of 10.01.2000.
V. A. Shkurupiy, Yu. N. Kurunov, and N. N. Yakovchenko, Lysosomotropism: Problems of Cell Physiology and Medicine [in Russian], Novosibirsk (1999).
V. A. Shkurupiy, V. A. Krasnov, E. P. Gulyaeva, et al., Radiat. Phys. Chem., 63, 691–695 (2002).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Suppl. 1, pp. 39–41, 2008
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Shkurupiy, V.A., Tsyrendorzhiev, D.D., Kurilin, V.V. et al. Effect of Oxidixed Dextrans on Oxidative and Metabolic Function of Mouse Peritoneal Macrophages In Vitro and In Vivo . Bull Exp Biol Med 146, 723–725 (2008). https://doi.org/10.1007/s10517-009-0390-9
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DOI: https://doi.org/10.1007/s10517-009-0390-9