We studied the possibility of population of macroporous agarose cryogel sponges by mesenchymal stromal bone marrow cells with their subsequent adipogenic differentiation. After 7-day culturing of mesenchymal stromal cells in agarose cryogel, the level of cell proliferation was 35%. After 3-week culturing in a medium inducing adipogenesis we observed accumulation of intracellular neutral lipids positively stained with Oil Red O. These findings can be used for the development of bioengineering constructions of the adipose tissue on the basis of spongy carriers.
Similar content being viewed by others
References
N. V. Latsinik, A. G. Grosheva, A. N. Narovlyanskii, et al., Byull. Eksp. Biol. Med., 137, No. 3, 257–384 (1987).
V. I. Lozinsky, L. G. Damshkaln, F. M. Plieva, et al., Byul. Izobret., 2004. No. 1. Pat. RF No. 2220987 (2001).
V. I. Lozinsky, Uspekhi Khim., 71, No. 6, 559–585 (2002).
K. Bloch, V. I. Lozinsky, I. Yu. Galaev, et al., J. Biomed. Mater. Res., 75A, No. 4, 802–809 (2005).
J. S. Boo, Y. Yamada, Y. Okazaki, et al., J. Craniofac. Surg., 13, 231–239 (2002).
H. Gloeckner, T. Jonuleit, and H. D. Lemke, J. Immunol. Methods, 252, Nos. 1–2, 131–138 (2001).
K. Hemmrich, D. von Heimburg, R. Rendchen, et al., Biomaterials, 26, No. 34, 7025–7037 (2005).
B. Johnstone, T. M. Hering, A. I. Caplan, et al., Exp. Cell Res., 238, 265–272 (1998).
T. C. Kononas, L. P. Bucky, C. Hurley, and J. W. Amy, J. Plast. Reconstr. Surg., 91, 763–768 (1993).
A. Loebsack, K. Greene, S. Wyatt, et al., J. Biomed. Mater. Res., 57, No. 4, 575–581 (2001).
C. W. Jr. Patrick, Anat. Rec., 263, 361–366 (2001).
C. W. Jr. Patrick, B. Zheng, and C. Johnston, Tissue Eng., 8, No. 2, 283–293 (2002).
Yu. A. Petrenko, N. A. Gorokhova, E. N. Tkachova, and A. Yu. Petrenko, Ukr. Biokhim. Zh., 77, No. 5, 100–105 (2005).
M. S. Stosich and J. J. Mao, Plast Reconstr Surg., 119, No. 1, 71–83 (2007).
D. von Heimburg, M. Kuberka, R. Rendchen, et al., Int. J. Artif. Organs., 26, No. 12, 1064–1076 (2003).
W. Wang, Y. Gu, and Y. Tabata, Transplantation, 73, 122–129 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 141–144, August, 2008
Rights and permissions
About this article
Cite this article
Petrenko, Y.A., Petrenko, A.Y., Damshkaln, L.G. et al. Growth and adipogenic differentiation of mesenchymal stromal bone marrow cells during culturing in 3D macroporous agarose cryogel sponges. Bull Exp Biol Med 146, 129–132 (2008). https://doi.org/10.1007/s10517-008-0236-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-008-0236-x