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CD24 and CD70 as differential markers of human hepatocellular carcinoma cells in culture

  • Oncology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

We developed a new method for evaluation of the purity of cell population in primary cultures of hepatocellular carcinoma. Expression of potential surface molecular markers on primary cultures of renal tumors was assayed by the method of flow cytofluorometry. CD24 and CD70 were identified as differential markers, which allowed us to distinguish cancer cells from tumor stromal cells in vitro. A negative marker CD90 was expressed on fibroblasts of the tumor stroma, but not on cancer cells. Combined use of these three markers allows evaluation of the severity of tumor culture contamination with stromal cells.

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References

  1. I. G. Aliaev and A. A. Krapivin, Renal Tumors [in Russian], Moscow (2002).

  2. A. Yu. Lupatov, P. A. Karalkin, Yu. G. Suzdal’tseva, et al., Kletochn. Tekhnol. Biol. Med., No. 4, 212–217 (2006).

  3. J. Diegmann, K. Junker, I. F. Loncarevic, et al., Neoplasia, 11, 933–938 (2006).

    Article  Google Scholar 

  4. T. Ebert, N. H. Bander, C. L. Finstad, et al., Cancer Res., 50, 5531–5536 (1990).

    PubMed  CAS  Google Scholar 

  5. K. Junker, W. Hindermann, F. von Eggeling, et al., J. Urol., 173, 2150–2153 (2005).

    Article  PubMed  CAS  Google Scholar 

  6. S. C. Lim and S. H. Oh, Pathol. Res. Pract., 201, 479–486 (2005).

    Article  PubMed  CAS  Google Scholar 

  7. J. G. Park, H. K. Oie, P. H. Sugarbaker, et al., Cancer Res., 47, 6710–6718 (1987).

    PubMed  CAS  Google Scholar 

  8. H. Sasano and T. Suzuki, Biomed. Pharmacother., 59, Suppl. 2, 334–336 (2005).

    Article  Google Scholar 

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Authors and Affiliations

  1. V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow

    P. A. Karalkin, N. E. Kovtun, A. Yu. Lupatov & K. N. Yarygin

  2. Russian State Medical University, Moscow

    P. A. Karalkin, A. Yu. Lupatov & K. N. Yarygin

  3. Urological Clinic, I. M. Sechenov Moscow Medical Academy, Moscow

    R. V. Kurynin, M. E. Chalyi & I. G. Aliaev

Authors
  1. P. A. Karalkin
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  2. N. E. Kovtun
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  3. A. Yu. Lupatov
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  4. R. V. Kurynin
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  5. M. E. Chalyi
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  6. I. G. Aliaev
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  7. K. N. Yarygin
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Corresponding author

Correspondence to P. A. Karalkin.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 12, pp. 668–671, December, 2007

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Karalkin, P.A., Kovtun, N.E., Lupatov, A.Y. et al. CD24 and CD70 as differential markers of human hepatocellular carcinoma cells in culture. Bull Exp Biol Med 144, 817–820 (2007). https://doi.org/10.1007/s10517-007-0439-6

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  • DOI: https://doi.org/10.1007/s10517-007-0439-6

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