Abstract
The structure of sarcoplasmic reticulum membranes was studied in the presence of modeled transmembrane Ca2+ gradient corresponding to the status of Ca2+ depot at different stages of the muscle contraction-relaxation cycle in health and disease. Various sites of the membrane were characterized using spectral analysis of tryptophan, pyrene, and merocyanine-540 fluorescence without evaluating specific changes in the molecules of membrane components (Ca2+-ATPase, ryanodine receptor, and lipids). The transmembrane Ca2+ gradient modulates the protein-lipid interactions and structural characteristics of the membrane. The proposed model can be used for studies of the effects of pharmacologically active substances and endogenous regulators.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 11, pp. 517–521, November, 2007
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Vekshina, O.M., Kim, Y.A. & Vekshin, N.L. Effect of Ca2+ gradient on the structure of sarcoplasmic reticulum membranes. Bull Exp Biol Med 144, 677–680 (2007). https://doi.org/10.1007/s10517-007-0403-5
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DOI: https://doi.org/10.1007/s10517-007-0403-5