Abstract
Changes in the immunocytochemical status of the nucleoli during long-term (6–8 months) in vitro culturing of HeLa (carcinoma of the cervix uteri) cells were described using new A3 monoclonal antibodies selectively reacting with human cell nucleoli. The appearance of cells with abnormal location of A3 antigen was paralleled by a significant increase of culture sensitivity to some external factors (protein synthesis inhibition and oxidative stress). The data indicate that location of one of the nucleolar antigens is an indicator of the qualitative status of HeLa cells in the culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. V. Domnina, O. Yu. Ivanova, B. V. Chernyak, et al., Biokhimiya, 67, No. 7, 890–900 (2002).
O. O. Zharskaya and O. V. Zatsepina, Tsitologiya, 47, No. 9, 780–788 (2005).
O. Yu. Pletyushkina, E. K. Fetisova, K. G. Lyamzaev, et al., 71, No. 1, 75–84 (2006).
O. Yu. Smirnova, V. A. Mishina, and O. V. Zatsepina, Tsitologiya, 45, No. 12, 1179–1187 (2003).
M. Chen and A. von Mikecz, Ann. N. Y. Acad. Sci., 1051, 382–389 (2005).
N. N. Dergunova, T. I. Bulycheva, E. G. Artemenko, et al., Immunol. Lett., 83, No. 1, 67–72 (2002).
M. S. Iordanov, D. Pribnow, J. L. Magun, et al., Mol. Cell Biol., 17, No. 6, 3373–3381 (1997).
A. Kageyama, I. Kusano, T. Tamura, et al., Biosci. Biotechnol. Biochem., 66, No. 4, 835–839 (2002).
S. Kito, Y. Morimoto, T. Tanaka, et al., J. Oral Pathol Med., 34, No. 8, 478–485 (2005).
C. P. Rubbi and J. Milner, EMBO J., 22, No. 22, 6068–6077 (2003).
A. Torres-Montaner, J. Bolivar, A. Astola, et al., J. Histochem. Cytochem., 48, No. 11, 1521–1530 (2000).
O. V. Zatsepina, R. Voit, I. Grummt, et al., Chromosoma, 102, No. 9, 599–611 (1993).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 9, pp. 321–324, September, 2007
Rights and permissions
About this article
Cite this article
Grigoryev, A.A., Zharskaya, O.O., Bulycheva, T.I. et al. Changes in the status of nucleolus during long-term culturing of human HeLa cells. Bull Exp Biol Med 144, 345–348 (2007). https://doi.org/10.1007/s10517-007-0329-y
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-007-0329-y