Abstract
The effects of short-term (3 h) and long-term (18 h) repeated hypoxic exposure (5% O2) on viability of endothelial cells, expression of adhesion molecules, and secretion of IL-6 and vascular endothelial growth factor were studied. The resultant number of apoptotic and necrotic cells indicates that cultured endothelium well tolerates repeated changes in partial oxygen pressure in the medium, despite the stress reaction manifesting in enhanced secretion of IL-6. Changes in number of cells carrying ICAM-1 on their surface and activation of the synthesis of vascular endothelial growth factor during longterm exposure attest to activation of angiogenesis and inhibition of apoptosis.
Similar content being viewed by others
References
A. S. Antonov, A. V. Krushinskii, and M. A. Nikolaeva, Tsitologiya, 23, No. 10, 1154–1160 (1981).
S. Akira, T. Hirano, T. Taga, and T. Kishimoto, FASEB J., 4, No. 11, 2860–2867 (1990).
M. H. Ali, S. A. Schlidt, N. S. Chandel, et al., Am. J. Physiol., 227, No. 5, Pt. 1, L1057–L1065 (1999).
N. Baudry, G. Danialou, J. Boczkowski, and E. Vicaut, Am. J. Respir. Crit. Care Med., 158, No. 2, 477–483 (1998).
A. M. Bowcock, A. Ray, H. A. Erlich, and P. B. Sehgal, Ann. N. Y. Acad. Sci., 557, 345–352 (1989).
N. S. Chandel, E. Maltepe, E. Goldwasser, et al., Proc. Natl. Acad. Sci. USA, 95, No. 20, 11 715–11 720 (1998).
J. M. Clark, Undersea Hyperb. Med., 21, No. 3, 251–264 (1994).
N. Ferrara and W. J. Henzel, Biochem. Biophys. Res. Commun., 161, No. 2, 851–858 (1989).
N. Ferrara, K. Houck, I. Jakeman, and D. E. Leung, Endocr. Rev., 13, No. 1, 18–32 (1992).
N. Ferrara, Recent Prog. Horm. Res., 55, 15–35 (2000).
J. A. Forsythe, B. H. Jiang, N. V. Iyer, et al., Mol. Cell Biol., 16, No. 9, 4604–4613 (1996).
M. A. Gimbrone Jr., R. S. Cotran, and J. Folkman, J. Cell Biol., 60, No. 3, 673–684 (1974).
J. M. Gleadle and P. J. Ratcliffe, Mol. Med. Today, 4, No. 3, 122–129 (1998).
K. J. Kim, B. Li, K. Houck, et al., Growth Factors, 7, No. 1, 53–64 (1992).
J. Kroll and J. Waltenberger, J. Biol. Chem., 272, No. 51, 32,521–32,527 (1997).
C. Michiels, E. Minet, D. Mottet, and M. Raes, Free Radic. Biol. Med., 33, No. 9, 1231–1242 (2002).
C. Michiels, T. Arnould, and J. Remacle, Cell Adhes. Commun., 5, No. 5, 367–374 (1998).
S. Ogawa, R. Shreeniwas, C. Butura, et al., Adv. Exp. Med. Biol., 281, 303–312 (1990).
D. J. Pinsky, S. F. Yan, C. Lawson, et al., Semin. Cell Biol., 6, No. 5, 283–294 (1995).
V. A. Tkachuk, L. V. Buravkova, T. J. Resink, et al., Rus. J. Physiol., 83, Nos. 5–6, 94–118 (1997).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 147–153, July, 2007
Rights and permissions
About this article
Cite this article
Grinakovskaya, O.S., Andreeva, E.R. & Buravkova, L.B. Effects of hypoxic gas mixtures on viability, expression of adhesion molecules, migration, and synthesis of interleukins by cultured human endothelial cells. Bull Exp Biol Med 144, 130–135 (2007). https://doi.org/10.1007/s10517-007-0272-y
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-007-0272-y