Abstract
In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.
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Koo, HY., Millay, M., Kume, T. (2015). Induction of Hypoxia in Vascular Endothelial Cell Culture. In: Slevin, M., McDowell, G. (eds) Handbook of Vascular Biology Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9716-0_3
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DOI: https://doi.org/10.1007/978-94-017-9716-0_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-9715-3
Online ISBN: 978-94-017-9716-0
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