Skip to main content
SpringerLink
Log in

Comparative study of postradiation genotoxic changes in mammalian cells by biochemical and cytogenetic methods

  • Biophysics and Biochemistry
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The results of studying the genotoxic effect of radiation on endometrial cells of irradiated rats using comet assay correlated with the index of spontaneous DNA degradation. Studying the genotoxic effect of in vitro irradiation (2 Gy) on human lymphocytes by means of chromosome analysis and fluorometric assay of nucleoids DNA in irradiated leukocytes also yielded similar results. Measuring of the biochemical marker holds much promise as an express method of evaluation of the genotoxic effect of radiation on mammalian somatic cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. M. Bershtein, E. V. Tsyrlina, T. E. Poroshina, et al., Byull. Eksp. Biol. Med., 132, No. 8, 200–204 (2001).

    Google Scholar 

  2. I. E. Vorobtsova, A. V. Semenov, A. Yu. Kanaeva, et al., Radiats. Biol. Radioekol., 40, No. 6, 645–650 (2000).

    CAS  Google Scholar 

  3. S. D. Ivanov, E. G. Kovan’ko, I. G. Popovich, and M. A. Zabezhinskii, Ibid., 39, No. 4, 418–424 (1999).

    CAS  Google Scholar 

  4. P. R. Cook and I. A. Brazell, Eur. J. Biochem., 84, 465–477 (1978).

    Article  PubMed  CAS  Google Scholar 

  5. C. Helma, P. Eckl, E. Gottmann, et al., Environ. Sci. Technol., 32, 1799–1805 (1998).

    Article  CAS  Google Scholar 

  6. S. Ivanov, L. Korytova, B. Yamshanov, et al., J. Exp. Clin. Cancer Res., 16, 413–418 (1997).

    PubMed  CAS  Google Scholar 

  7. N. Kopjar and V. Garaj-Vrhovac, Int. J. Hyg. Environ. Health, 208, 179–191 (2005).

    Article  PubMed  CAS  Google Scholar 

  8. S. C. Short, S. Bourne, C. Martindale, et al., Radiat. Res., 164, 292–302 (2005).

    Article  PubMed  CAS  Google Scholar 

  9. S. Tsuda, Y. Kosaka, M. Murakami, et al., Mutat. Res., 415, 191–200 (1998).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central Research Institute of Roentgenoradiology, Russia

    S. D. Ivanov, V. A. Yashmanov, E. G. Kovan’ko & I. E. Vorobtsova

  2. N. N. Petrov Institute of Oncology, Russian Ministry of Health, St. Petersburg

    T. E. Poroshina & L. M. Bershtein

Authors
  1. S. D. Ivanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. A. Yashmanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. G. Kovan’ko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. E. Vorobtsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. E. Poroshina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. M. Bershtein
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

__________

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 142, No. 12, pp. 635–638, December, 2006

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ivanov, S.D., Yashmanov, V.A., Kovan’ko, E.G. et al. Comparative study of postradiation genotoxic changes in mammalian cells by biochemical and cytogenetic methods. Bull Exp Biol Med 142, 679–682 (2006). https://doi.org/10.1007/s10517-006-0449-9

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-006-0449-9

Key Words

Search

Navigation