Abstract
The two-spotted spider mite (TSSM), Tetranychus urticae, is a chelicerate herbivore with a wide host range and strong ability to develop pesticide resistance. Experimental TSSM populations are easy to maintain, and the recent publication of the complete TSSM genome sequence and development of RNA interference-based reverse genetics protocols make this species an ideal chelicerate model for the study of pesticide resistance and plant–herbivore interactions. In such studies, treated leaf discs are often used for oral delivery of test compounds. When preparing these leaf discs, the organosilicone surfactant Silwet L-77 is used to promote wetting of the leaf surface and distribution of the test compound across the entire leaf surface. Here, we examined the toxicity of Silwet L-77 and found it to be toxic to TSSMs. We then developed a novel means of preparing leaf discs in which a polypropylene sheet rather than Silwet L-77 was used to ensure distribution of a tracer dye across the entire leaf surface. These leaf discs were then successfully used to deliver the tracer dye into the midgut of TSSMs. No significant differences were observed in the survival, fecundity, or feeding activity of TSSMs fed on leaf discs treated with water via our novel method compared with those fed on untreated leaf discs. Thus, our novel method of preparing leaf discs eliminates concerns regarding the bioactivity of surfactants in TSSMs, and we anticipate that it will be useful for improving oral delivery-based bioassays that use TSSMs.
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References
Bensoussan N, Santamaria ME, Zhurov V et al (2016) Plant-herbivore interaction: dissection of the cellular pattern of Tetranychus urticae feeding on the host plant. Front Plant Sci 7:1105. https://doi.org/10.3389/fpls.2016.01105
Bensoussan N, Zhurov V, Yamakawa S et al (2018) The digestive system of the two-spotted spider mite, Tetranychus urticae (Koch), in the context of the mite-plant interaction. Front Plant Sci 9:1206. https://doi.org/10.3389/FPLS.2018.01206
Bosse TC, Veerman A (1996) Involvement of vitamin A in the photoperiodic induction of diapause in the spider mite Tetranychus urticae is demonstrated by rearing an albino mutant on a semi-synthetic diet with and without p-carotene or vitamin A. Physiol Entomol 21:188–192. https://doi.org/10.1111/j.1365-3032.1996.tb00854.x
Bryon A, Kurlovs AH, Dermauw W et al (2017) Disruption of a horizontally transferred phytoene desaturase abolishes carotenoid accumulation and diapause in Tetranychus urticae. Proc Natl Acad Sci USA 114:E5871–E5880. https://doi.org/10.1073/pnas.1706865114
Cazaux M, Navarro M, Bruinsma KA et al (2014) Application of two-spotted spider mite Tetranychus urticae for plant–pest interaction studies. J Vis Exp. https://doi.org/10.3791/51738
Chandler LD (1995) Effect of surfactants on beet armyworm and fall armyworm larvae, 1994. Arthropod Manag Tests 20:353
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743. https://doi.org/10.1046/j.1365-313x.1998.00343.x
Cowles RS, Cowles EA, McDermott AM, Ramoutar D (2000) “Inert” formulation ingredients with activity: toxicity of trisiloxane surfactant solutions to twospotted spider mites (Acari: Tetranychidae). J Econ Entomol 93:180–188. https://doi.org/10.1603/0022-0493-93.2.180
Dermauw W, Wybouw N, Rombauts S et al (2013) A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae. Proc Natl Acad Sci USA 110:E113–E122. https://doi.org/10.1073/pnas.1213214110
Fire A, Xu S, Montgomery MK et al (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811
Gotoh T, Korenaga T, Ikejima S, Hoshino T (2008) Acaricide-mediated suppression of Panonychus citri (Mcgregor) (Acari: Tetranychidae) in pear orchards in Japan. Int J Acarol 34:393–402
Grbic M, Van Leeuwen T, Clark RM et al (2011) The genome of Tetranychus urticae reveals herbivorous pest adaptations. Nature 479:487–492. https://doi.org/10.1038/nature10640
Imai T, Tsuchiya S, Fujimori T (1995) Aphicidal effects of Silwet L-77, organosilicone nonionic surfactant. Appl Entomol Zool 30:380–382. https://doi.org/10.1303/aez.30.380
Jonckheere W, Dermauw W, Zhurov V et al (2016) The salivary protein repertoire of the polyphagous spider mite Tetranychus urticae: a quest for effectors. Mol Cell Proteom 15:3594–3613
Kantaratanakul S, Rodriguez JG (1979) Nutritional studies in Tetranychus urticae (KOCH) (acarina, tetranychidae). I. Development of a chemically defined diet. Int J Acarol 5:83–92. https://doi.org/10.1080/01647957908683130
Khila A, Grbić M (2007) Gene silencing in the spider mite Tetranychus urticae: dsRNA and siRNA parental silencing of the distal-less gene. Dev Genes Evol 217:241–251. https://doi.org/10.1007/s00427-007-0132-9
Kwon DH, Park JH, Lee SH (2013) Screening of lethal genes for feeding RNAi by leaf disc-mediated systematic delivery of dsRNA in Tetranychus urticae. Pestic Biochem Physiol 105:69–75. https://doi.org/10.1016/J.PESTBP.2012.12.001
Kwon DH, Park JH, Ashok PA et al (2016) Screening of target genes for RNAi in Tetranychus urticae and RNAi toxicity enhancement by chimeric genes. Pestic Biochem Physiol 130:1–7. https://doi.org/10.1016/J.PESTBP.2015.11.005
Kwon DH, Park JH, Lee SH (2017) Identification of initial responsive genes to systemic dsRNA ingestion in the two-spotted spider mite, Tetranychus urticae Koch. J Asia Pac Entomol 20:229–235. https://doi.org/10.1016/J.ASPEN.2017.01.006
Li G, Niu J-Z, Zotti M et al (2017) Characterization and expression patterns of key ecdysteroid biosynthesis and signaling genes in a spider mite (Panonychus citri). Insect Biochem Mol Biol 87:136–146. https://doi.org/10.1016/J.IBMB.2017.06.009
Liao C-Y, Xia W-K, Feng Y-C et al (2016) Characterization and functional analysis of a novel glutathione S-transferase gene potentially associated with the abamectin resistance in Panonychus citri (McGregor). Pestic Biochem Physiol 132:72–80. https://doi.org/10.1016/J.PESTBP.2015.11.002
Mota-Sanchez D, Wise JC (2017) Arthropod pesticide resistance database. http://www.pesticideresistance.org. Accessed 10 Sept 2017
Piraneo TG, Bull J, Morales MA et al (2015) Molecular mechanisms of Tetranychus urticae chemical adaptation in hop fields. Sci Rep 5:17090
Purcell MF, Schroeder WJ (1996) Effect of Silwet L-77 and diazinon on three tephritid fruit flies (Diptera: Tephritidae) and associated endoparasitoids. J Econ Entomol 89:1566–1570
R Core Team (2016) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
Salama HS, Rasmy AH (1971) Artificial feeding of mites and its use in screening acaricides. Zeitschrift für Angew Entomol 67:31–33. https://doi.org/10.1111/j.1439-0418.1971.tb02092.x
San Miguel K, Scott GJ (2015) The next generation of insecticides: dsRNA is stable as a foliar-applied insecticide. Pest Manag Sci 72:801–809. https://doi.org/10.1002/ps.4056
Shapiro JP, Schroeder WJ, Stansly PA (1998) Bioassay and efficacy of Bacillus thuringiensis and an organosilicone surfactant against the citrus leafminer (Lepidoptera: Phyllocnistidae). Fla Entomol 81:201–210
Shi L, Zhang J, Shen G et al (2015) Silencing NADPH-cytochrome P450 reductase results in reduced acaricide resistance in Tetranychus cinnabarinus (Boisduval). Sci Rep 5:15581
Shi L, Wei P, Wang X et al (2016) Functional analysis of esterase TCE2 gene from Tetranychus cinnabarinus (Boisduval) involved in acaricide resistance. Sci Rep 6:18646
Srinivasan R, Hoy MA, Singh R, Rogers ME (2008) Laboratory and field evaluations of Silwet L-77 and kinetic alone and in combination with imidacloprid and abamectin for the management of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae). Fla Entomol 91:87–100. https://doi.org/10.1653/0015-4040(2008)091%5B0087:LAFEOS%5D2.0.CO;2
Suzuki T, España MU, Nunes MA et al (2017a) Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae. PLoS One 12:e0180658. https://doi.org/10.1371/journal.pone.0180658
Suzuki T, Nunes MA, España MU et al (2017b) RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: a comparative analysis of five methods for gene silencing. PLoS One 12:e0180654. https://doi.org/10.1371/journal.pone.0180654
Tipping C, Bikoba V, Chander GJ, Mitcham EJ (2003) Efficacy of Silwet L-77 against several arthropod pests of table grape. J Econ Entomol 96:246–250. https://doi.org/10.1603/0022-0493-96.1.246
Van Leeuwen T, Vontas J, Tsagkarakou A et al (2010) Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: a review. Insect Biochem Mol Biol 40:563–572. https://doi.org/10.1016/J.IBMB.2010.05.008
van der Geest LPS, Bosse TC, Veerman A (1983) Development of a meridic diet for the two-spotted spider mite Tetranychus urticae. Entomol Exp Appl 33:297–302
Walling MV, White DC, Rodriguez JG (1968) Characterization, distribution, catabolism, and synthesis of the fatty acids of the two-spotted spider mite, Tetranychus urticae. J Insect Physiol 14:1445–1458
Xia W-K, Shen X-M, Ding T-B et al (2016) Functional analysis of a chitinase gene during the larval–nymph transition in Panonychus citri by RNA interference. Exp Appl Acarol 70:1–15. https://doi.org/10.1007/s10493-016-0063-0
Xu Z, Liu Y, Wei P et al (2017a) High gama-aminobutyric acid contents involved in abamectin resistance and predation, an interesting phenomenon in spider mites. Front Physiol 8:216. https://doi.org/10.3389/fphys.2017.00216
Xu Z, Wu Q, Xu Q, He L (2017b) Functional analysis reveals glutamate and gamma-aminobutyric acid-gated chloride channels as targets of avermectins in the carmine spider mite. Toxicol Sci 155:258–269
Zhu Y-X, Song Y-L, Huang H-J et al (2018) Comparative analyses of salivary proteins from the facultative symbiont-infected and uninfected Tetranychus truncatus. Syst Appl Acarol 23:1027–1042. https://doi.org/10.11158/saa.23.6.3
Acknowledgements
This study was supported by JSPS KAKENHI Grant nos. 16K18661 and 18H02203 to TS. TS was also supported by the Joint Study Program of Ajinomoto Co., Inc. HGA was supported by a Mitsubishi Corporation International Scholarship. NAG was supported by JSPS Invitational Fellowships for Research in Japan.
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Abouelmaaty, H.G., Fukushi, M., Abouelmaaty, A.G. et al. Leaf disc-mediated oral delivery of small molecules in the absence of surfactant to the two-spotted spider mite, Tetranychus urticae. Exp Appl Acarol 77, 1–10 (2019). https://doi.org/10.1007/s10493-018-0335-y
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DOI: https://doi.org/10.1007/s10493-018-0335-y