Abstract
The occurrence of Suidasia medanensis (= S. pontifica) mites in Malaysian house dust was first reported in 1984. The taxonomy of this storage mite is, however, quite confusing. Therefore, we need an accurate identification to resolve morphological problems due to its minute size and some overlapping characters between species. The purpose of this study was to demonstrate the application of partial mitochondrial cytochrome c oxidase subunit I (COI) sequences for the identification of S. medanensis by PCR. Identity of the mite was first determined by observing morphological characters under a light microscope. Genomic DNA of S. medanensis mites was successfully extracted prior to PCR and DNA sequencing using COI universal primers. The length of the COI sequences obtained was 378 bp. BLAST analysis of amplicon sequences showed that local S. medanensis COI region had 99% maximum identity with S. medanensis nucleotide sequence (AY525568) available in the GenBank. As the phylogenetic tree generated indicated, COI sequences from this study were clustered with S. medanensis from Korea and the UK in one major clade, supported with high bootstrap value (> 85%). Results of the phylogenetic analysis of this COI gene were congruent with the morphological identification and provided strong support for a single clade of local S. medanensis.
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Introduction
Families of mites that have been identified in house dust are Pyroglyphidae, Acaridae, Glycyphagidae, Chortoglyphidae, Suidasiidae and Echimyopodidae (Puerta et al. 2005; Cui 2014). Amongst these, Suidasiidae is one of the most important families due to its economic and public health impact. Of this family, the genus Suidasia comprises 18 species (Krantz et al. 2009), which are commonly found in house dust and stored food products in tropical countries such as India, Taiwan and Thailand (Chmielewski 2009). Suidasia medanensis (= S. pontifica) Oudemans (Acari: Astigmata: Suidasiidae) was reported for the first time in Malaysia in house dust samples in 1984 (Ho and Nadchatram 1984). The mites were widely distributed as such and they were reported to infest many stored products including grains, cheese, nuts, milk powder and rice grain and flour (Ho and Nadchatram 1985; Ho and Mariana 1994; Ho 1996; Mariana et al. 2000, 2010).
The species involved is of medical significance as it has the ability to produce potent allergens causing allergic reactions in humans (Chmielewski 2009; Mariana et al. 2011). Whole-body extract of S. medanensis has at least 14 allergens, suggesting an important allergenic load and sensitizing potential (Puerta et al. 2005). To determine the clinical relevance of S. medanensis, some studies have shown a high percentage of asthmatics patients to be sensitized to the mite species (Mariana et al. 2002; Yap et al. 2014). Furthermore, ingestion of food prepared from flour contaminated with these mites was reported to cause anaphylaxis with symptoms of breathlessness and facial angiodema to the allergic patients (Sanchez-Borges et al. 1997, 2009).
The quality of mite allergens is depending on the production of body proteins and digestive enzymes that are discharged within fecal pellets (Calderon et al. 2015). Accurate species identification is important to avoid contamination of other foreign mites for standardization of the allergen production (Frati et al. 2012). Dust mites have been identified mainly based on morphological characteristics of the adult stage. However, it is difficult to identify species by their phenotypic traits alone due to their microscopic size (Li et al. 2015) and similarities of characters (Beroiz et al. 2014). There is also no figure available for descriptions of morphological polymorphism in adults (Vargas et al. 2005). Moreover, immature mites often lack obvious diagnostic characters (Li et al. 2010) for species confirmation and they often must be reared until adult stage for accurate identification. The taxonomy of the genus Suidasia is somewhat confusing and the identification of species was unresolved due to variability of morphological characters that overlap between closely related species (Mercado et al. 2001).
An accurate molecular tool for species identification would be highly valuable. Progress in molecular biology techniques has enabled the identification and the determination by DNA sequence analysis of phylogenetic relationships of a range of mite species. The central part of the mitochondrial cytochrome c oxidase subunit I (COI) region is highly conserved and has often been used for identification of astigmatid mites and for determination of their intra- and interspecific variation. This is due to their variation in the COI sequences and higher rates of molecular evolution between closely related mite and tick species (Yang et al. 2011; Erster et al. 2013; Lv et al. 2014; Khaing et al. 2014). The purpose of the present study was to demonstrate the application of partial mitochondrial COI sequences for identifying S. medanensis by PCR. Genetic profile of this mite was also discussed based on molecular phylogeny using the COI region. Information on the genetics of domestic mites is important to understand the population dynamics of laboratory cultures and in mite control.
Materials and methods
Culture of mites
Suidasia medanensis mites were first obtained from house dust sample collected from Pahang, Malaysia. Those colonies were established in the 1980’s and maintained in the Acarology Unit, Institute for Medical Research, Malaysia. Colonies consisting of mixed stages of mites were maintained at 25 ± 2 °C and 75 ± 2% relative humidity. Purity of the colonies was checked by microscopical examination of a few randomly mounted specimens. The identity of mites was determined by analyzing external morphological characteristics (Hughes 1976; Fain and Philips 1978) using a phase contrast microscope (Nikon, Japan).
Harvest of mites and DNA extraction
The mite cultures were harvested with a ‘heat escape’ method in which mites migrated from the sieve located on top of a funnel to a 1-ml sterile collection Eppendorf tube at its bottom. The collected bodies (ca. 100 mg) were immediately resuspended in 25 µl of phosphate buffered saline (PBS) and 75 µl of animal tissue lysis (ATL) buffer. The mites were then crushed using sterile plastic tips for 5 min prior to adding 20 µl of Proteinase K. The following steps were performed using the manufacturer’s supplementary protocol for the Qiagen DNeasy® Kit for animal tissues (Qiagen, Germany). Purity and concentration of DNA was determined using Thermo Scientific NanoDrop™ 1000 spectrophotometer (Wilmington, DE, USA). The extracted DNA was either directly used as a template for PCR amplification or stored at − 20 °C until further usage.
PCR amplification
Amplification of the COI gene region was performed using the following universal primers: forward (5′-GTT TTG GGA TAT CTC TCA TAC-3′) and reverse (5′-GAG CAA CAA CAT AAT AAG TAT-3′) which amplified the central region of COI. PCR was performed in 50 µl total volume containing 10 µl of DNA, 25 µl of 2X Taq PCR Master Mix (Qiagen), 2.5 µl of each primer (10 µM), and 10 µl nuclease-free water. For each PCR reaction, a negative control containing sterile double distilled water and a positive control containing mite DNA (A. ovatus) were included. The mixture was amplified by the following conditions: initial denaturation at 94 °C for 3 min followed by 35 cycles denaturation at 94 °C for 30 s, annealing at 50 °C for 30 s, elongation at 72 °C for 1 min and a final elongation step at 72 °C for 7 min. PCR was carried out in an Eppendorf Mastercycler Personal machine. Amplicons were analyzed by 1.5% agarose gel electrophoresis and positive bands were viewed under an ultraviolet trans-illuminator (at 254 nm wavelength).
DNA sequencing
The DNA fragment was excised from the gel using a sterile sharp gel cutter and purified using 5 Prime PCR Agarose Gelextract Mini Kit (Hamburg, Germany) according to the manufacturer’s protocol. All PCR products were then sent for DNA sequencing to First Base Laboratory, Malaysia. The sequencing was bi-directional for all specimens and the primer combination for this step was the same as that used in the PCR amplification.
Gene sequence analysis
Multiple sequence alignment was carried out using the Clustal-W program of BioEdit (Hall 2005). Identified sequences were compared with available sequences of dust mite previously deposited in GenBank databases using the Basic Local Alignment Search Tool (BLAST) program. In addition, two S. medanensis sequences that were available in GenBank (Accession Nos. AY525568 and KJ820779) were aligned simultaneously as analysis background and species control. In this analysis, Tyrophagus putrescentiae (GenBank Accession No. EF527826) was selected as an outgroup for COI gene.
Phylogenetic analysis
Phylogenetic analyses were performed using Molecular Evolutionary Genetic Analysis (MEGA) software v.4.0 with the neighbor-joining (NJ) method. Bootstrap values were obtained from 1000 replicates. Sequence divergence was calculated using MEGA among taxa, based on Kimura-2-parameter (K2P) distance.
Results
The morphology of S. medanensis can be distinguished from other mite species by the presence of a dorsal transverse groove that divides the propodosoma from the hysterosoma (Fig. 1a). The ventral part of the mite is finely wrinkled or covered with scale-like cuticle (Fig. 1b). Their legs are stout with claw at the end of the tarsus (Fig. 1b). The molecular analysis matched its morphological identification. The primer combination of COI-F and COI-R was demonstrated to amplify mtDNA of the mite. Length of the obtained PCR product of the COI was approximately 378 bp in all examined samples (Fig. 2). The BLAST analysis of amplicon sequences indicated that the COI region of the local S. medanensis had 99% sequence similarity with the S. medanensis (AY525568) available in the GenBank.
In total 327 bp fragments were obtained from the multiple COI gene alignments (Fig. 3). Sequence analysis indicated that 48 (14.6%) variable sites were detected within the COI gene and four (8.3%) characters were parsimony informative. Additionally, the conserved sites were constituted by 279 (85.3%) characters showing that the COI segment is a very conserved gene in the mtDNA. In this study, the amino acids asparagine (Asn), histidine (His) and lysine (Lys) were not detected in any of the Suidasia sequences (Fig. 4). Leucine (Leu) was the most abundant (> 43%) amino acid in all S. medanesis samples.
The phylogenetic tree of COI indicated that S. medanensis from Korea and UK were first apart from the Malaysian mites with a 95% highly significant bootstrap value (Fig. 5). A monophyletic clade with 85% bootstrap value was observed for the local species.
Discussion
The microscopic mite identification has been addressed as a difficult task with the main limitation being the need for expert taxonomic personnel. Moreover, due to their minute size, immature stages often have indistinguishable characters for species identification and they often must be reared to adult stage to be accurately identified. More recently, the availability of DNA sequence data of various mites in international databases has opened the door for simpler molecular-based species identification. In the present study, PCR has successfully produced partial COI sequences of the local S. medanensis mites. The COI sequences obtained in this study have been used for species validation of mites as a complement for morphological identification. The results were in concordance to several studies which reported that COI gene has been often used for molecular identification of dust mites and other astigmatid mites (Navajas et al. 1996; Yang et al. 2011; Khaing et al. 2014).
This study underscores that DNA barcoding using the COI gene can be used to categorize species of dust mites at molecular level. Our results indicated that there was little variation in amino acid percentage of the COI gene between local S. medanensis and those from other parts of the world. This may be due to variation of base composition (deletion or insertion) that occurs mostly through synonymous transitions (Navajas et al. 1996). However, it presumes that the genotypic characterization of the COI gene does not have detectable effects on the phenotypic traits of S. medanensis possibly due to the much higher conservation of the length of COI sequences (Khaing et al. 2014).
The NJ analysis of the COI gene positioned the local S. medanensis in the phylogenetic tree according to their morphological identification. The families Acaridae (T. putrescentiae) and Suidasiidae (S. medanensis) were distinguished clearly by formation of two major clades. Our study also showed the nearest relatives and provided strong support for a single clade of the local mite. Intraspecific genetic distances were relatively smaller (data not shown) for the COI sequence of both local sequences. Therefore, and because of the limited sample size of the study, intraspecific sequence variation could be investigated further, particularly for those species collected from the field and for species found in other countries worldwide.
In general, the PCR assay can be directly applied in purified mite fractions for a diagnosis or treatment of dust mite allergy, especially when the availability of intact adult mites for morphological identification is limited. Although a single PCR assay may take longer to perform than a morphological identification, the PCR technique has great advantages in terms of turn-around time that can easily identify higher numbers of specimens in a single operation. The present results support that the COI sequence is a reliable molecular marker for identification and analysis of the phylogenetic relationship of storage mites.
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We would like to thank the Director General of Health Malaysia for his permission to publish this article.
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Ernieenor, F.C.L., Ernna, G., Jafson, A.S. et al. PCR identification and phylogenetic analysis of the medically important dust mite Suidasia medanensis (Acari: Suidasiidae) in Malaysia. Exp Appl Acarol 76, 99–107 (2018). https://doi.org/10.1007/s10493-018-0285-4
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DOI: https://doi.org/10.1007/s10493-018-0285-4