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Ciceribacter sichuanensis sp. nov., a plant growth promoting rhizobacterium isolated from root nodules of soybean in Sichuan, China

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Abstract

The fast-growing rhizobia-like strains S101T and S153, isolated from root nodules of soybean (Glycine max) in Sichuan, People’s Republic of China, underwent characterization using a polyphasic taxonomy approach. The strains exhibited growth at 20–40 °C (optimum, 28 °C), pH 4.0–10.0 (optimum, pH 7.0) and up to 2.0% (w/v) NaCl (optimum, 0.01%) on Yeast Mannitol Agar plates. The 16S rRNA gene of strain S101T showed 98.4% sequence similarity to the closest type strain, Ciceribacter daejeonense L61T. Major cellular fatty acids in strain S101T included summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. The predominant quinone was ubiquinone-10. The polar lipids of strain S101T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethyl ethanolamine, phosphatidyl ethanolamine, amino phospholipid, unidentified phosphoglycolipid and unidentified amino-containing lipids. The DNA G + C contents of S101T and S153 were 61.1 and 61.3 mol%, respectively. Digital DNA–DNA hybridization relatedness and average nucleotide identity values between S101T and C. daejeonense L61T were 46.2% and 91.4–92.2%, respectively. In addition, strain S101T promoted the growth of soybean and carried nitrogen fixation genes in its genome, hinting at potential applications in sustainable agriculture. We propose that strains S101T and S153 represent a novel species, named Ciceribacter sichuanensis sp. nov., with strain S101T as the type strain (= CGMCC 1.61309 T = JCM 35649 T).

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Acknowledgements

We thank Prof. (Emeritus) Aharon Oren (The Hebrew University of Jerusalem, Jerusalem 9190401, Israel) for the suggestion about species epithet.

Funding

This work was supported by the National Key Research and Development Program of China (2022YFD1901402), the Science and Technology Program of Sichuan Province (2023NSFSC0138, 2022ZHXC0007) and the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202308).

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  1. Yanqin Zhang and Yuanxue Chen contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Yanqin Zhang, Yuanxue Chen, Petri Penttinen, Ying Quan, Licheng Wen, Miao Yang, Xiaoping Zhang, Qiang Chen, Lingzi Zhang & Kaiwei Xu

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Xing Wang & Xiaoxia Zhang

  3. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, 450000, People’s Republic of China

    Junjie Zhang

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  1. Yanqin Zhang
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Contributions

KX and YC conceived and designed the experiments. KX supervised the experiments. YC, YZ, KX, XZ and JZ contributed to discussion of the results, and writing and revising the manuscript. YZ, YQ, LW and MY performed the experiments and analyzed data. XZ, QC and LZ participated in collecting the closely related type strains from China Agricultural University. Revisions and editing: PP. All authors contributed to writing the article.

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Correspondence to Xiaoxia Zhang or Kaiwei Xu.

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Zhang, Y., Chen, Y., Penttinen, P. et al. Ciceribacter sichuanensis sp. nov., a plant growth promoting rhizobacterium isolated from root nodules of soybean in Sichuan, China. Antonie van Leeuwenhoek 117, 46 (2024). https://doi.org/10.1007/s10482-024-01941-5

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