Abstract
An endophytic actinobacterium, designated strain HUAS 5T, was isolated from the root tissue of Cathaya argyrophylla collected in Chenzhou city of Hunan Province, PR China. This strain produced grey aerial mycelium that differentiated into spiral spore chains with spiny-surfaced ellipsoidal spores on Gause's synthetic No. 1 medium. Strain HUAS 5T grew well on Gause's synthetic No. 1, Reasoner'2 and ISP serial media. This strain grew at 15–40 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 7.0) and in presence of 0–5.0% (w/v) NaCl. The predominant cellular fatty acids of strain HUAS 5T (> 5.0%) were iso-C16:0, iso-C14:0, anteiso-C15:0, iso-C15:0, C16:0, iso-C16:1 H and Sum in Feature 3 (C16:1 ω7c/C16:1 ω6c). Sequence analysis of the 16S rRNA gene indicated that this strain belonged to the genus Streptomyces and exhibited highest sequence similarity to Streptomyces hirsutus NRRL B-2713T (97.3%), which is much less than 98.7% cut-off point of species definitions for bacteria and archaea. Phylogenetic analysis of 16S rRNA gene sequence and whole genome indicated that strain HUAS 5T formed an independent lineage, which suggested that it belonged to a potential novel species. Based on the morphological, cultural, physio-biochemical properties and chemotaxonomy, strain HUAS 5T (= MCCC 1K08552T = JCM 36055T) is deemed to represent a novel Streptomyces species, for which we put forward the name Streptomyces cathayae sp. nov.
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Abbreviations
- MCCC:
-
Marine Culture Collection of China
- ISP:
-
International Streptomyces Project
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Acknowledgements
The authors thank Wangjie Xu and Shuang Wan (Wuhan Benagen Technology Co., Ltd, Hubei, China), Zhisheng Zhu, Xiupian Liu and Li Gu (Marine Culture Collection of China, MCCC, Xiamen, China) for providing excellent technical assistance. Many thanks to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his help in the naming of microorganisms.
Funding
This research was funded by the project of Hunan Provincial Education Department (23B0659), the Hunan Natural Science Foundation (2023JJ30436, 2022JJ30420, 2023JJ50325), the National Natural Science Foundation of China (31972835), Central guidance local science and technology development fund projects (2023ZYC012), Innovation Team of Microbial Technology in Hunan University of Arts and Science (202026).
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LL and YW contributed to conception and design of the study. CW, RNH, ZYC, YSL and PX performed the statistical analysis. PM and CW wrote the first draft of the manuscript. RNH, ZYC, YSL, PX, LL and YW assisted with investigation and sampling. All authors contributed to manuscript revisions, read and approved the submitted version.
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Mo, P., Wu, C., Han, R. et al. Streptomyces cathayae sp. nov., an endophytic actinobacterium from the root tissue of Cathaya argyrophylla. Antonie van Leeuwenhoek 117, 31 (2024). https://doi.org/10.1007/s10482-024-01927-3
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DOI: https://doi.org/10.1007/s10482-024-01927-3