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Streptomyces Spinosirectus sp. nov., Isolated From the Medicinal Plant Xanthium Sibiricum

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Abstract

A novel actinobacterium, designated strain CRSS-Y-16T, was isolated from the healthy leaves of Xanthium sibiricum, in China and characterized by a polyphasic approach. This strain produced abundant aerial mycelia that generated rod-shaped spores with spiny surfaces. The cell wall contained ll-diaminopimelic acid. The major cellular fatty acids (>10.0%) were C16:0, iso-C16:0, and C18:1 ω9c. The predominant menaquinones were MK-9(H2) and MK-9(H4). The detected polar lipids were diphosphatidylglycerol, hydroxyl phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannoside, phospholipids of unknown structure containing glucosamine and unidentified phospholipids. The genomic G+C content was 70.7%. The full-length 16S rRNA gene sequence analysis indicated that strain CRSS-Y-16T belonged to the genus Streptomyces and shared <98.7% sequence similarities with all recognized type species of the genus. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain formed a distinct branch. Phylogenomic analysis demonstrated that it was closely related to Streptomyces panaciradicis 1MR-8T. However, the clustering patterns resulting from phylogenomic tree verified that strain CRSS-Y-16T represented a novel Streptomyces species. This result was further confirmed by low average nucleotide identity and digital DNA–DNA hybridization values (87.54% and 30.1%) between them. Based on all these data, it is concluded that strain CRSS-Y-16T represents a novel Streptomyces species, for which the name Streptomyces spinosirectus sp. nov. is proposed. The type strain is CRSS-Y-16T (=MCCC 1K06950T=JCM 35007T).

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Data Availability

All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

The authors are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

Funding

This work was supported by the Hunan Provincial Natural Science Foundation of China and Xiangtan Science and Technology Bureau (2022JJ50125) and Scientific Research Project of Hunan Province Department of Education (20A200).

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Authors and Affiliations

  1. School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan, 411201, People’s Republic of China

    Yin-Feng Wang, Si-Ren Hu, Li Fu, Yan Xiao, Xin-Ke Tang & Jian Gao

  2. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan, 411201, People’s Republic of China

    Jian Gao

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Contributions

YFW and SRH carried out the polyphasic taxonomy, data analysis and wrote the manuscript. LF and XKT prepared figures and tables. YX provided the samples. JG corrected and reviewed the paper and takes full responsibility for the final submission. All authors reviewed and approved the final version of the paper.

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Correspondence to Jian Gao.

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Wang, YF., Hu, SR., Fu, L. et al. Streptomyces Spinosirectus sp. nov., Isolated From the Medicinal Plant Xanthium Sibiricum. Curr Microbiol 80, 27 (2023). https://doi.org/10.1007/s00284-022-03134-7

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