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Devosia lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic Microcystis

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Abstract

A novel alphaproteobacterial strain JXJ CY 41T was isolated from a culture mass of Microcystis, collected from Lake Dianchi, south-west, China. Strain JXJ CY 41T was gram-strain-negative, aerobic, motile, with rod-shaped cells (0.4–1.0 × 1.7–3.5 μm). It was positive for catalase and starch hydrolysis, negative for oxidase and hydrolysis of Tweens (20, 40, and 80). Growth occurred at 10–44 °C, pH 5.0–10.0, and 0–5.0% (w/v) NaCl. Major fatty acids included C16:0 (28.1%), 11-methyl C18:1 ω7c (36.7%) and C18:1 ω7c (20.8%). Q10 was the sole ubiquinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid, and an unidentified lipid. The DNA G + C content was 63.1%. Its 16S rRNA gene sequence showed high similarities with Devosia oryziradicis G19T (99.5%; not validly published), D. yakushimensis Yak96BT (98.3%) and D. ginsengisoli Gsoil 520T (98.1%), and less than 98.1% similarities with other members of the genus Devosia. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain JXJ CY 41T and its 5 closest similar strains were 19.9–24.1% and 75.7–80.5%, respectively. Based on the data above, strain JXJ CY 41T was identified as a novel species of the genus Devosia, for which the epithet Devosia lacusdianchii sp. nov. was proposed. The type strain is JXJ CY 41T (= KCTC 72812T = CGMCC 1.17502T). Strain JXJ CY 41T exhibited different interactions with Microcystis aeruginosa FACHB-905 (Maf) under different conditions, and Maf could control the bacterial cellular density by secreting unknown specific chemical compounds according to its nutritional requirements.

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Funding

This research was supported by the National Natural Science Foundation of China (No. 32360028).

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  1. Qing-Lin Deng and Yao Xiao have contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy and Life Science, Jiujiang University, Jiujiang, 332000, People’s Republic of China

    Qing-Lin Deng, Yao Xiao, Ru Jia, Xin Xie, Le Wang, Xin Wang, Jian-Yuan Yang, Qi-Gen Guo & Bing-Huo Zhang

  2. Jiujiang Key Laboratory for the Development and Utilization of Traditional Chinese Medicine Resources in Northwest Jiangxi, Jiujiang, People’s Republic of China

    Bing-Huo Zhang

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  1. Qing-Lin Deng
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Contributions

BZ designed the experiments; QD, YX, RJ, XX, LW, and XW performed the experiments; QD, YX, QG and BZ analysed the data; JY and BZ drafted and revised the manuscript. All authors have read and approved the final version of the manuscript.

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Deng, QL., Xiao, Y., Jia, R. et al. Devosia lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic Microcystis. Antonie van Leeuwenhoek 117, 12 (2024). https://doi.org/10.1007/s10482-023-01909-x

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