Abstract
A rare fungal endophyte, identified as Buergenerula spartinae (C28), was isolated from the roots of Cymbidium orchids and was characterised and evaluated for its antimicrobial activities. Bio-guided fractionation revealed 4 fractions from B. spartinae (C28) having antibacterial activities against at least one bacterial pathogen tested (Bacillus cereus and Staphylococcus aureus). However, inhibitory activities were absent against pathogenic fungi (Ganoderma boninense, Pythium ultimum and Fusarium solani). Fraction 2 and fraction 4 of B. spartinae (C28) exhibited potent antibacterial activities against S. aureus (MIC: 0.078 mg/mL) and B. cereus (MIC: 0.313 mg/mL), respectively. LCMS analysis revealed the presence of antibacterial agents and antibiotics in fraction 2 (benoxinate, pyropheophorbide A, (−)-ormosanine and N-undecylbenzenesulfonic acid) and fraction 4 (kaempferol 3-p-coumarate, 6-methoxy naphthalene acetic acid, levofuraltadone, hinokitiol glucoside, 3-α(S)-strictosidine, pyropheophorbide A, 5′-hydroxystreptomycin, kanzonol N and 3-butylidene-7-hydroxyphthalide), which may be responsible for the antibacterial activities observed. Most of the bioactive compounds profiled from the antibacterial fractions were discovered for the first time from endophytic isolates (i.e. from B. spartinae (C28)). Buergenerula spartinae (C28) from Cymbidium sp. is therefore, an untapped resource of bioactive compounds for potential applications in healthcare and commercial industries.
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The data that support the findings of this study are available from the corresponding author, upon reasonable request.
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Fig. S1 Antagonistic activity of Buergenerula spartinae (C28) against (a) Ganoderma boninense, (b) Pythium ultimum and (c) Fusarium solani detected by the dual culture assay. Control plates were established to observe the growth of (d) G. boninense, (e) P. ultimum and (f) F. solani in the absence of B. spartinae (C28)
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Chua, R.W., Song, K.P. & Ting, A.S.Y. Comparative analysis of antimicrobial compounds from endophytic Buergenerula spartinae from orchid. Antonie van Leeuwenhoek 116, 1057–1072 (2023). https://doi.org/10.1007/s10482-023-01870-9
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DOI: https://doi.org/10.1007/s10482-023-01870-9