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Microvirga splendida sp. nov., bacteria isolated from soil

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Abstract

Two bacterial strains, BT325T and BT690, were isolated from soil samples collected in Korea. Both strains were Gram stain-negative, short rod-shaped, and formed light-pink colored colonies. The 16S rRNA sequence similarity of strains BT325T and BT690 shared a sequence similarity of 99.7%. Both strains shared the highest 16S rRNA gene similarity of 98.6% with Microvirga arabica SV2184PT, followed by Microvirga ossetica V5/3 M T (98.5% and 98.2%, respectively), Microvirga soli R491T (98.3% and 98.2%, respectively), Microvirga aerilata (98.2% and 98.08%, respectively), Microvirga makkahensis (98.08% and 97.8%, respectively). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BT325T and BT690 were positioned in a distinct lineage within the family Methylobacteriaceae (order Rhizobiales, class Alphaproteobacteria). The genome size of strain BT325T was 5,200,315 bp and the genomic DNA G + C content was 64.3 mol%. The sole respiratory quinone of strain BT325T was Q-10 and the predominant cellular fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Polyphasic taxonomic analysis of biochemical, chemotaxonomic, and phylogenetic analyses suggested that strains BT325T represents a novel bacterial species within the genus Microvirga, for which the name Microvirga splendida is proposed. The type strain of Microvirga splendida is BT325T (= KCTC 72406 T = NBRC 114847 T).

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Abbreviations

R2A:

Reasoner’s 2A

KACC:

Korean agricultural culture collection

NA:

Nutrient agar

LBA:

Laked Blood Agar

TSA:

Tryptic Soy Agar

MAC:

MacConkey

PGAP:

Prokaryotic genome annotation pipeline

ANI:

Average nucleotide identity

DDH:

In silico DNA–DNA hybridization

NJ:

Neighbor-joining

ML:

Maximum–likelihood

MP:

Maximum–parsimony

HPLC:

High performance lipid chromatography

TLC:

Thin layer chromatography

FAME:

Fatty acid methyl esters

MIS:

Microbial identification system

CDS:

Coding genes

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanolamine

PC:

Phosphatidylcholine

L:

Unidentified polar lipid

AL:

Unidentified aminolipid

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Acknowledgements

We are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

Funding

This work was supported by a research grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002203), by a research grant from Seoul Women’s University (2022-0320), and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1G1A110144).

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Authors and Affiliations

  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 01797, Korea

    Yuna Park, Soohyun Maeng, Tuvshinzaya Damdintogtokh, Hyejin Oh, Minji Bang & Myung Kyum Kim

  2. Division of Applied Food System, Major in Food Science & Technology, Seoul Women’s University, Seoul, 01797, Korea

    Jaewoo Bai

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  1. Yuna Park
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  2. Soohyun Maeng
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  3. Tuvshinzaya Damdintogtokh
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All authors equally contributed in this work.

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The GenBank accession numbers for the 16S rRNA gene sequences of strains BT325T and BT690 are MT795758 and MW463443, respectively. The whole-genome sequences of strain BT325T have been deposited into DDBJ/EMBL/GenBank under the accession number JAELXT000000000.

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Park, Y., Maeng, S., Damdintogtokh, T. et al. Microvirga splendida sp. nov., bacteria isolated from soil. Antonie van Leeuwenhoek 115, 741–747 (2022). https://doi.org/10.1007/s10482-022-01715-x

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